Synthesis of diazo functionalized solid supports and their application towards the enrichment of phosphorylated peptides.
Synthesis of diazo functionalized solid supports and their application towards the enrichment of phosphorylated peptides.
复制标题
重氮功能化固体支持物的合成及其在磷酸化肽富集方面的应用。
DOI：
10.1039/b906577f
复制
发表时间：
2009
影响因子：
3.2
通讯作者：
Tepe,JetzeJ
中科院分区：
文献类型：
--
作者：
FrawleyCass,SamanthaM;Reid,GavinE;Tepe,JetzeJ
We describe herein the synthesis and application of a range of diazo-functionalized materials as platforms for the solid phase enrichment of phosphorylated peptides from protein digests. Diazo-functionalized solid phase resins are employed to bind covalently and specifically with the phosphate moiety of a peptide substrate, allowing for non-phosphorylated peptides to be efficiently removed by filtration. The technique described herein is compatible with enrichment of phospho-serine, threonine and tyrosine residues and was used to successfully enrich phosphorylated substrates from β-casein and ovalbumin digests in low-picomolar quantities.
