Tissue apoptosis in mice infected with Leptospira interrogans serovar Icterohaemorrhagiae.
Tissue apoptosis in mice infected with Leptospira interrogans serovar Icterohaemorrhagiae.
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DOI：
10.1186/s40409-015-0022-y
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Cardoso TC
中科院分区：
文献类型：
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作者：
Marinho M;Táparo CV;Oliveira-Júnior IS;Perri SH;Cardoso TC
本研究旨在探讨感染后2、4、7、10、14和21 d时疑问钩端螺旋体血清型黄疸出血引起的BALB/c幼龄小鼠的一些凋亡特征的发生。将动物安乐死，取肺、肝、肾进行组织病理学分析和caspase-3抗原免疫组化检测。在肾和肝组织中观察到染色质凝结，但在肺组织中没有观察到。Caspase-3反应细胞主要表现为肾上皮细胞，与第2、4、7天相比，第14、21天Caspase-3反应细胞水平较高（p = 0.025; p < 0.05）。肺切片显示感染后第10、14天caspase-3标记肺泡细胞高于感染后第7天（p = 0.0497; p < 0.05）。肝脏切片显示，与感染后2、4、7和10天相比，感染后14和21天的活性细胞水平最高（p = 0.0069; p < 0.05）。本研究结果表明，在感染过程的后期，感染乳酸菌可诱导肾脏、肝脏和肺部通过caspase-3依赖途径激活细胞凋亡。
This investigation aimed to evaluate the occurrence of some apoptotic features induced by Leptospira interrogans serovar Icterohaemorrhagiae infection in young BALB/c mice during 2, 4, 7, 10, 14 and 21 days post-infection (dpi). The animals were euthanized and lung, liver and kidneys were harvested to histopathology analysis and immunohistochemistry to caspase-3 antigen detection was performed. Chromatin condensation in kidney and liver tissues, but not in lung tissue, was observed. Caspase-3 reactive cells, mainly characterized as renal epithelial cells, were detected in the days 14 and 21 at high levels when compared to days 2, 4 and 7 (p = 0.025; p < 0.05). Lung sections revealed caspase-3 labeled alveolar cells in 10 and 14 days post-infection was higher than observed at 7 days (p = 0.0497; p < 0.05). Liver sections demonstrated reactive cells at a highest level at 14 and 21 days post-infection when comparison to 2, 4, 7 and 10 days (p = 0.0069; p < 0.05). Our results suggest that infection of L. interrogans induce in kidney, liver and lung an activation of apoptosis mediated by caspase-3 dependent pathway in later phases of infectious process.