Epidermal growth factor receptor is a common element in the signaling pathways activated by cell volume changes in isosmotic, hyposmotic or hyperosmotic conditions
Epidermal growth factor receptor is a common element in the signaling pathways activated by cell volume changes in isosmotic, hyposmotic or hyperosmotic conditions
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DOI：
10.1007/s11064-005-8837-5
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发表时间：
2005-12-01
影响因子：
4.4
通讯作者：
Pasantes-Morales, H
中科院分区：
文献类型：
--
作者：
Lezama, R;Díaz-Téllez, A;Pasantes-Morales, H
体外渗透压的变化，包括高渗或低渗条件，都会引起一些酪氨酸激酶受体(TKR)的酪氨酸磷酸化。我们发现表皮生长因子受体(EGFR)被细胞膨胀(低渗、等渗尿素、高渗山梨醇)或收缩(高渗氯化钠或棉子糖)激活，并讨论了这些明显相反的条件达到相同效果的机制，即EGFR激活。有证据表明，这是由于整合蛋白、p38和Src家族的酪氨酸激酶的早期激活所致，它们都在两种不等渗条件下激活。整合素和p38对TKR的反式激活可能是通过对金属蛋白酶的作用而发生的。在这篇综述中讨论的信息指出，TKR是渗透转导中的元件，由于它们作为许多细胞内信号通路的汇聚点，TKR是放大和多样化对非等渗和细胞体积变化的初始反应的有用机制。连接到TKR的各种效应通路有利于细胞应对细胞体积的变化，包括对应激的适应、细胞骨架重塑、黏附反应、细胞存活以及最终恢复原始细胞体积的适应机制。
Changes in external osmolarity, including both hyper- or hyposmotic conditions, elicit the tyrosine phosphorylation of a number of tyrosine kinase receptors (TKR). We show here that the epidermal growth factor receptor (EGFR) is activated by both cell swelling (hyposmolarity, isosmotic urea, hyperosmotic sorbitol) or shrinkage (hyperosmotic NaCl or raffinose) and discuss the mechanisms by which these apparently opposed conditions come to the same effect, i.e., EGFR activation. Evidence suggests that this results from early activation of integrins, p38 and tyrosine kinases of the Src family, which are all activated in the two anisosmotic conditions. TKR transactivation by integrins and p38 is likely occurring via an effect on the metalloproteinases. Information discussed in this review, points to TKR as elements in osmotransduction as a useful mechanism to amplify and diversify the initial response to anisosmolarity and cell volume changes, due to their privileged situation as convergence point for numerous intracellular signaling pathways. The variety of effector pathways connected to TKR is advantageous for the cell to cope with the changes in cell volume including adaptation to stress, cytoskeleton remodeling, adhesion reactions, cell survival and the adaptive mechanisms to ultimately restore the original cell volume.