GENERATION OF LONG-LIVED B-CELLS IN GERM-FREE MICE
GENERATION OF LONG-LIVED B-CELLS IN GERM-FREE MICE
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DOI：
10.1002/eji.1830210732
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发表时间：
1991-07-01
影响因子：
5.4
通讯作者：
RAJEWSKY, K
中科院分区：
文献类型：
--
作者：
FORSTER, I;MULLER, W;RAJEWSKY, K
在本研究中，我们已经解决的问题，是否组成的外周B细胞池是在传统的，特定的病原体或无菌饲养条件下饲养的小鼠相似。 为此目的，通过流式细胞术定量各种B谱系亚群的代表性，并通过在体内用胸苷类似物5-溴-2 '-脱氧尿苷标记分裂细胞来分析B细胞的寿命。 结果表明，除了骨髓中CD 45 R（B220）钝B谱系细胞的分数增加和无菌小鼠中脾B细胞的周转略快外，不同种类小鼠中B细胞的代表性和周转没有明显差异。 数据表明，即使在没有微生物刺激的情况下，也会产生稳定的外周B细胞库。
In the present study we have addressed the question of whether the composition of the peripheral B cell pool is similar in mice raised under conventional, specific pathogen-free or germ-free breeding conditions. For this purpose, the representation of various B lineage subsets was quantitated by flow cytometry and the lifespan of B cells analyzed by labeling dividing cells with the thymidine analogue 5-bromo-2'-deoxyuridine in vivo. The results indicate that except for an increased fraction of CD45R(B220)dull B lineage cells in the bone marrow and a marginally faster turnover of splenic B cells in germ-free compared to conventional mice, there is no obvious difference in the representation and turnover of B cells in the different kinds of mice. The data indicate that a stable pool of peripheral B cells is generated even in the absence of microbial stimulation.