HUNDREDS OF ANKYRIN-LIKE REPEATS IN FUNCTIONALLY DIVERSE PROTEINS - MOBILE MODULES THAT CROSS PHYLA HORIZONTALLY
HUNDREDS OF ANKYRIN-LIKE REPEATS IN FUNCTIONALLY DIVERSE PROTEINS - MOBILE MODULES THAT CROSS PHYLA HORIZONTALLY
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DOI：
10.1002/prot.340170405
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发表时间：
1993-12-01
期刊：
影响因子：
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通讯作者：
BORK, P
中科院分区：
文献类型：
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作者：
BORK, P
基于模式搜索和系统的数据库筛选，几乎650个不同的锚定样（ANK）重复，从几乎所有的门已被确定，其中150多个在这里首次报道。它们在功能多样的蛋白质如酶、毒素和转录因子中的存在强烈表明结构域改组，但它们在原核生物和酵母中的出现排除了外显子改组。其传播机制尚不清楚，但至少在三个案例中，似乎涉及水平基因转移。ANK重复序列至少有四个连续的拷贝。末端重复序列在序列上更易变。内部重复序列的一个特征是预测的中央疏水α-螺旋，其可能与其他重复序列相互作用。锚定样重复序列的功能与蛋白质-蛋白质相互作用中的作用相容。 (C)1993 Wiley-Liss，Inc.
Based on pattern searches and systematic database screening, almost 650 different ankyrin-like (ANK) repeats from nearly all phyla have been identified; more than 150 of them are reported here for the first time. Their presence in functionally diverse proteins such as enzymes, toxins, and transcription factors strongly suggests domain shuffling, but their occurrence in prokaryotes and yeast excludes exon shuffling. The spreading mechanism remains unknown, but in at least three cases horizontal gene transfer appears to be involved. ANK repeats occur in at least four consecutive copies. The terminal repeats are more variable in sequence. One feature of the internal repeats is a predicted central hydrophobic alpha-helix, which is likely to interact with other repeats. The functions of the ankyrin-like repeats are compatible with a role in protein-protein interactions. (C) 1993 Wiley-Liss, Inc.