Distribution of lipids and apolipoproteins in human plasma by vertical spin ultracentrifugation.
Distribution of lipids and apolipoproteins in human plasma by vertical spin ultracentrifugation.
复制标题
通过垂直旋转超速离心法测定人血浆中脂质和载脂蛋白的分布。
DOI：
10.1016/0003-9861(86)90022-6
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发表时间：
1986
影响因子：
3.9
通讯作者：
McConathy,WJ
中科院分区：
文献类型：
--
作者：
Campos,E;McConathy,WJ
本文用单自旋垂直超离心法研究了人血浆中各种脂蛋白的分离。对通过单次旋转垂直超离心获得的组分进行详细的载脂蛋白（A-I、A-II、B、C-III和E）和脂质（胆固醇、胆固醇酯和三酰甘油）分析。ApoB主要位于低密度脂蛋白（LDL）部分，而Lp（a）在延伸到高密度脂蛋白区域的部分中检测到。ApoA-I和ApoA-II的分布在个体间存在差异，表明HDL组成存在异质性，这一点通过ApoC-III、ApoD和ApoE的分布得到证实。ApoC-III和ApoD主要与HDL 3相关，而ApoE在受试者中表现出最大的变异性，分布在整个密度谱中。胆固醇和胆固醇酯的分布与主要载脂蛋白A-I和B的洗脱一致，而三酰甘油的分布是可变的。卵磷脂：胆固醇酰基转移酶（LCAT）的免疫测定表明，大多数LCAT存在于高密度脂蛋白至极高密度脂蛋白区域，但LCAT也检测到低密度脂蛋白和免疫反应性延伸到极低密度脂蛋白区域。载脂蛋白A-I和A-II以及中间脂蛋白密度区（LDL 1）中的LCAT的存在与代表脂解残留物的这些物质一致。这项研究表明，快速（3小时）单次旋转垂直超速离心可以深入了解脂蛋白的异质性，并为监测血浆脂质转运系统的扰动提供了有用的工具。结合免疫吸附、免疫测定和微量脂质分析，该程序提供了分离和表征各种脂蛋白亚种的方法。
A study on the fractionation of human plasma into various lipoprotein classes was performed by single spin vertical ultracentrifugation. Detailed apolipoprotein (A-I, A-II, B, C-III, and E) and lipid (cholesterol, cholesterol ester, and triacylglycerol) analyses were performed on the fractions obtained by single spin vertical ultracentrifugation. ApoB was located primarily in the low-density lipoprotein (LDL) fraction whereas Lp(a) was detected in the fractions extending into the high-density lipoprotein region. The distributions of ApoA-I and ApoA-II varied among individuals, suggesting heterogeneity in the composition of HDL, and this was confirmed by the distributions of ApoC-III, ApoD, and ApoE. ApoC-III and ApoD were associated primarily with HDL3while ApoE showed the most variation among subjects, spreading across the entire density spectrum. Distributions of cholesterol and cholesterol ester coincided with the elution of major apolipoproteins A-I and B while the distribution of triacylglycerol was variable. An immunoassay for lecithin:cholesterol acyltransferase (LCAT) demonstrated that the majority of LCAT was present in the high- to very high-density lipoprotein region, but LCAT was also detected in LDL and the immunoreactivity extended into the very low-density lipoprotein region. The presence of apolipoproteins A-I and A-II, as well as LCAT in the intermediate lipoprotein density region (LDL1) was consistent with these species representing lipolytic remnants. This study indicated that rapid (3 h) single spin vertical ultracentrifugation offers insights into the heterogeneity of lipoproteins and offers a useful tool for monitoring perturbations of the plasma lipid transport system. In conjunction with immunosorbers, immunoassays, and micro lipid analyses, this procedure offers a method to isolate and characterize various lipoprotein subspecies.