Amplification for Whole Genome Sequencing of Bacteriophages from Single Isolated Plaques Using SISPA.
Amplification for Whole Genome Sequencing of Bacteriophages from Single Isolated Plaques Using SISPA.
复制标题
使用 SISPA 对单个分离斑块中的噬菌体进行全基因组测序扩增。
DOI：
10.1007/978-1-4939-7343-9_12
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Fouts,DerickE
中科院分区：
文献类型：
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作者：
Fouts,DerickE
Genomics has greatly transformed our understanding of phage biology; however, traditional methods of DNA isolation for whole genome sequencing have required phages to be grown to high titers in large-scale preparations, potentially selecting for only those phages that can grow efficiently under laboratory conditions. This may also select for mutations or deletions that enable more efficient growth in culture. The ability to sequence a bacteriophage genome from a single isolated plaque reduces these risks while decreasing the time and complexity of bacteriophage genome sequencing. A method of amplification and library preparation is described, utilizing Sequence Independent Single Primer Amplification (SISPA), that can be used for whole genome shotgun sequencing of bacteriophages from a single isolated plaque.
