Influence of estrogen on individual exercise motivation and bone protection in ovariectomized rats
Influence of estrogen on individual exercise motivation and bone protection in ovariectomized rats
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DOI：
10.1177/0023677218756455
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发表时间：
2018-10-01
影响因子：
2.4
通讯作者：
Bernhardt, Ricardo
中科院分区：
文献类型：
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作者：
Mueller, Sebastian T.;Keiler, Annekathrin M.;Bernhardt, Ricardo
骨骼保护和新陈代谢与雌激素水平直接相关，但运动也被认为具有骨骼保护作用。雌激素水平降低会导致各种疾病，例如骨质流失和运动驱动力降低。本研究的目的是探讨雌激素对个体自愿运动动机和骨保护的影响。我们研究了假手术，卵巢切除，卵巢切除雌激素补充Wistar大鼠（20周龄），无论是否获得运动轮。我们选择了一种实验方法，在这种方法中，我们可以通过皮下芯片的帮助来监测组养大鼠的个体运动，这些大鼠可以随意进入跑步轮。从第0周至第6周，每隔两周对胫骨进行体内和离体显微计算机断层扫描分析。此外，胫骨骨小梁结构进行了评价的基础上组织形态学分析。我们观察到E2具有显著的骨保护作用。对于运动表现，观察到相当高的组内变异性，尤其是在E2组。我们推测，占主导地位的行为发生在组内圈养的大鼠，导致在一个层次访问的运行轮和高度可变的距离运行。运动不能预防卵巢切除术引起的骨质流失。然而，卵巢切除大鼠体内雌激素的缺乏导致雌激素补充剂阻止的活性急剧降低。我们的研究结果对未来的研究具有重要意义。运动组内变异性高的原因需要在未来的研究中进行调查。
Bone protection and metabolism are directly linked to estrogen levels, but exercise is also considered to have bone protective effects. Reduced estrogen levels lead to a variety of disorders, for example, bone loss and reduced movement drive. The objective of this study was to investigate the effects of estrogen on individual voluntary exercise motivation and bone protection. We investigated sham operated, ovariectomized, and ovariectomized with estrogen supplemented Wistar rats (20 weeks old) either with or without access to exercise wheels. We selected an experimental approach where we could monitor the individual exercise of group-housed rats with ad libitum access to a running wheel with the help of a subcutaneous chip. In vivo and ex vivo microcomputed tomography analyses of the tibia were performed at two-week intervals from week 0 to week 6. Furthermore, tibial trabecular structure was evaluated based on histomorphometric analyses. We observed a significant bone protective effect of E2. For exercise performance, a substantially high intra-group variability was observed, especially in the E2 group. We presume that dominant behavior occurs within the group-housed rats resulting in a hierarchical access to the running wheel and a high variability of distance run. Exercise did not prevent ovariectomy-induced bone loss. However, lack of estrogen within the ovariectomized rats led to a drastically reduced activity prevented by estrogen supplementation. Our findings are important for future studies working with group-housed rats and exercise. The reason for the high intra-group variability in exercise needs to be investigated in future studies.