Lactobacillus plantarum CECT 7527, 7528 and 7529: probiotic candidates to reduce cholesterol levels
Lactobacillus plantarum CECT 7527, 7528 and 7529: probiotic candidates to reduce cholesterol levels
复制标题
DOI：
10.1002/jsfa.6467
复制
发表时间：
2014-03-15
影响因子：
4.1
通讯作者：
Cune, Jordi
中科院分区：
文献类型：
--
作者：
Bosch, Montserrat;Fuentes, Mari C.;Cune, Jordi
背景高胆固醇血症在冠状动脉疾病的发生和进展中起着关键作用。有许多药物可用于治疗这种疾病，但它们通常价格昂贵并且可能产生不良副作用。因此，对乳酸菌进行了筛选，以选择候选益生菌菌株来降低胆固醇水平。根据国际指南，在进行了多次体外测试以证明菌株的功能后，选择了三种植物乳杆菌菌株（CECT 7527、7528和7529）作为潜在的降低胆固醇水平的益生菌。结果这三种菌株在胃肠道条件下表现出较高的存活能力和粘附肠道细胞的能力。关于脂质代谢，这些菌株显示出大量的胆汁盐水解酶，特别是当组合时。此外，这些菌株直接从培养基中吸收胆固醇。培养基中存在的部分胆固醇通过结合到细菌细胞表面而被去除。最后，这三种菌株，尤其是 CECT 7529，产生大量的丙酸和丁酸。结论综合起来，这些特征表明这些菌株可能是降低高血液胆固醇水平的极好候选菌株。 (c) 2013年化学工业学会
BACKGROUNDHypercholesterolaemia plays a key role in the development and progression of coronary artery disease. There are numerous drugs available to treat this condition but they are often expensive and can have unwanted side effects. Therefore, a screening of lactic acid bacteria to select candidate probiotic strains to reduce cholesterol levels was carried out. Three Lactobacillus plantarum strains (CECT 7527, 7528 and 7529) were selected as potential probiotics to reduce cholesterol levels after conducting several in vitro tests for demonstrating the functionalities of the strains according to international guidelines.RESULTSThe three strains showed a high ability to survive under gastrointestinal tract conditions and to adhere to intestinal cells. Regarding lipid metabolism, the strains showed a great production of bile salt hydrolase, especially when combined. Moreover, the strains assimilated cholesterol directly from the medium. Part of the cholesterol present in the medium was removed via binding onto the bacterial cellular surface. Finally, the three strains, especially CECT 7529, produce large quantities of propionic and butyric acids.CONCLUSIONCombined, these characteristics suggest that these strains could be excellent candidates for reducing high blood cholesterol levels. (c) 2013 Society of Chemical Industry