Supramolecular Tandem Enzyme Assays
Supramolecular Tandem Enzyme Assays
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DOI：
10.1002/chem.201103364
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发表时间：
2012-03-01
影响因子：
4.3
通讯作者：
Nau, Werner M.
中科院分区：
文献类型：
--
作者：
Dsouza, Roy N.;Hennig, Andreas;Nau, Werner M.
我们概念化的一种新的方法对酶的测定的基础上的可逆性和竞争性结合的荧光染料和底物以及产品的酶反应的大环主机。这种方法被称为超分子串联分析，并已被应用于抑制剂和激活剂的筛选，传感器阵列的发展，和对映体过量测定氨基酸。通过荧光的简单和快速读出允许它们直接实施到高通量筛选中。
We conceptualize a novel approach towards enzyme assays based on the reversible and competitive binding of a fluorescent dye and the substrate as well as product of an enzymatic reaction to a macrocyclic host. This method was termed supramolecular tandem assay, and has been applied to inhibitor and activator screening, sensor array development, and enantiomeric excess determination of amino acids. The simple and rapid read-out by fluorescence allows their straightforward implementation into high-throughput screening.