Production of Polyvinyl Alcohol Oxidase by a Symbiotic Mixed Culture
Production of Polyvinyl Alcohol Oxidase by a Symbiotic Mixed Culture
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DOI：
10.1128/aem.44.1.28-32.1982
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发表时间：
1982-07
影响因子：
4.4
通讯作者：
M. Shimao;Yoshifumi Taniguchi;Shitsuu Shikata;N. Kato;C. Sakazawa
中科院分区：
文献类型：
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作者：
M. Shimao;Yoshifumi Taniguchi;Shitsuu Shikata;N. Kato;C. Sakazawa
假单胞菌生产聚乙烯醇(PVA)酶菌株VM15C是一株利用PVA共生的混合培养物的PVA降解菌，在不同的培养条件下进行了检测。尽管营养液中不存在PVA，但VM15C表现出与以PVA为唯一碳源培养的PVA氧化酶活性大致相同的产生率，而添加葡萄糖的产生率低于营养液或PVA培养。在营养肉汤培养中产生的PVA氧化酶活性主要存在于细胞中，并且大部分活性似乎在细胞质中。相反，在以PVA为唯一碳源的培养中，该活性在发酵液中的比例高于营养肉汤培养。因此，该菌株产生的PVA氧化酶活性是结构性的和可抑制的，尽管产生的活性的定位会因生长条件而改变。
Production of polyvinly alcohol (PVA) oxidase by Pseudomonas sp. strain VM15C, a PVA degrader of a symbiotic PVA-utilizing mixed culture, was examined in various cultures. Despite the absence of PVA in the culture in nutrient broth, VM15C showed approximately the same productivity of PVA oxidase activity as that in the culture with PVA as the sole carbon source, whereas the productivity in the culture with glucose was lower than that of either the nutrient broth or the PVA culture. PVA oxidase activity produced in the nutrient broth culture was predominantly present in the cells, and most of the activity appeared to be in the cytoplasm. In contrast, in the culture with PVA as the sole carbon source, the activity was present in the culture fluid in a larger ratio than in the nutrient broth culture. Thus, production of PVA oxidase activity by this strain was constitutive and repressible, although localization of the produced activity was changed by growth conditions.