Direct electrochemical detection of sodium azide in physiological saline buffers using highly boron-doped diamond electrodes
Direct electrochemical detection of sodium azide in physiological saline buffers using highly boron-doped diamond electrodes
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DOI：
10.1016/j.snb.2006.03.003
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发表时间：
2007-01
影响因子：
8.4
通讯作者：
Akane Suzuki;T. Ivandini;Akihide Kamiya;S. Nomura;Mikito Yamanuki;K. Matsumoto;A. Fujishima;Y. Einaga
中科院分区：
文献类型：
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作者：
Akane Suzuki;T. Ivandini;Akihide Kamiya;S. Nomura;Mikito Yamanuki;K. Matsumoto;A. Fujishima;Y. Einaga
通过在一些生理盐水缓冲溶液中使用高硼掺杂金刚石（BDD）电极来检查叠氮化钠的直接检测。研究了三种重要的盐水缓冲液：ADA 缓冲液（N-(2-乙酰氨基)亚氨基二乙酸）、EDTA 钠和咪唑。氢封端的 BDD 被固定用于研究，因为与氧封端的 BDD 相比，它对叠氮化物氧化表现出更好的敏感性。检查了宽范围的缓冲液浓度。在其与缓冲液的混合物中观察到充分分离的叠氮化钠峰，从而实现选择性叠氮化钠检测。电流响应恰好是叠氮化钠和缓冲液电流响应的总添加。事实证明，本方法可用于直接检测用于细胞计数的商业稀释剂样品中的叠氮化钠。采用标准添加法。两个样品中均检测到叠氮化钠浓度为 502 和 1355μgmL−1，RSD 分别为 0.07 和 2.37%。通过与制造商给出的样品浓度进行比较来实现验证。简单、选择性、良好的灵敏度和出色的可重复检测使得金刚石电极对于众多电分析应用非常有吸引力。
Direct detection of sodium azide was examined by using highly boron-doped diamond (BDD) electrodes in some physiological saline buffer solutions. Three important saline buffers, ADA buffer (N-(2-acetamido) iminodiacetic acid), sodium EDTA and imidazole, were investigated. Hydrogen-terminated BDD was fixed for the investigation since it showed much better sensitivity for azide oxidation in comparison with the oxygen-terminated one. Wide range of buffer concentration was examined. A well-separated peak of sodium azide was observed in its mixture with the buffers, resulting in selective sodium azide detection. The current responses were exactly total addition of sodium azide and buffer current responses. The present method was evidenced to be available for direct detection of sodium azide in two samples of commercial diluents for cell counting. A standard addition method was applied. Sodium azide concentrations of 502 and 1355μgmL−1were detected in both samples with the R.S.D.s of 0.07 and 2.37%, respectively. Validation was achieved by the comparison with the sample concentrations given by the manufacturer. Simple, selective, good sensitivity and excellent reproducible detection make the diamond electrodes very attractive for numerous electroanalytical applications.