Wheel running following spinal cord injury improves locomotor recovery and stimulates serotonergic fiber growth
Wheel running following spinal cord injury improves locomotor recovery and stimulates serotonergic fiber growth
复制标题
DOI：
10.1111/j.1460-9568.2007.05469.x
复制
发表时间：
2007-04-01
影响因子：
3.4
通讯作者：
Anderson, Aileen J.
中科院分区：
文献类型：
--
作者：
Engesser-Cesar, Christie;Ichiyama, Ronaldo M.;Anderson, Aileen J.
在脊髓损伤（SCI）后，通过手动步训练、机器人步训练或自主轮跑来进行锻炼是一种很有前景的治疗方法。动物模型为研究体育活动影响脊髓损伤恢复的机制提供了工具。在本研究中，我们扩展了先前的实验，表明损伤前和损伤后在平面跑步轮中跑步可以改善脊髓损伤后的恢复，并研究了恢复的机制。我们用伤后滚轮跑测试了一个临床相关的模型，在这个模型中，我们给小鼠提供了3天或7天/周的滚轮接触。在中度T9挫伤后15周的开放场地行为观察中，运动能力的改善在各组、久坐组、3天跑步组和7天跑步组中均有显著的线性增加。跑步机行走的运动学分析显示，与久坐对照组相比，每周3天和7天轮跑组的行走能力都有所提高。脊髓损伤节段神经元数量的体视定量显示各组间无差异。然而，使用各向同性虚拟平面的血清素免疫染色的立体定量显示，跑步组在病变尾部的血清素纤维长度增加。这些观察结果表明，功能的改善可能与损伤中心尾侧血清素纤维的变化有关。
Exercise, through manual step training, robotic step training or voluntary wheel running, is emerging as a promising therapy after spinal cord injury (SCI). Animal models provide a tool to investigate the mechanisms by which physical activity influences recovery from SCI. In the present study, we extend previous experiments showing improved recovery after SCI with both pre- and post-injury running in a flat-surface running wheel and investigate mechanisms of recovery. We tested a clinically relevant model using post-injury wheel running, in which we provided mice with access to wheels either 3 days or 7 days/week. Open field behavior, observed for 15 weeks following moderate T9 contusion injury, showed a significant linear increase in locomotor improvements across groups, sedentary, 3-day runners and 7-day runners. Kinematic analysis of treadmill walking revealed that both wheel-running groups, 3 and 7 days/week, improved stepping ability compared with sedentary controls. Stereological quantification of neuron number in the injured segment of the spinal cord revealed no differences between the groups. However, stereological quantification of serotonin immunostaining using isotropic virtual planes showed increases in serotonin fiber length caudal to the lesion in the running groups. These observations suggest that improvement in function may be related to changes in serotonin fibers immediately caudal to the injury epicenter.