Macrophage colony formation supported by purified CSF-1 and/or interleukin 3 in serum-free culture: evidence for hierarchical difference in macrophage colony-forming cells.
Macrophage colony formation supported by purified CSF-1 and/or interleukin 3 in serum-free culture: evidence for hierarchical difference in macrophage colony-forming cells.
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DOI：
10.1182/blood.v67.4.859.bloodjournal674859
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发表时间：
1986-04
期刊：
影响因子：
20.3
通讯作者：
K. Koike;E. Stanley;J. Ihle;M. Ogawa
中科院分区：
文献类型：
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作者：
K. Koike;E. Stanley;J. Ihle;M. Ogawa
使用无血清培养系统，我们研究了在纯化的CSF-1，白细胞介素3（IL 3）或两种因子的组合的存在下培养的骨髓细胞的小鼠巨噬细胞集落形成。CSF-1支持巨噬细胞和嗜中性粒细胞-巨噬细胞集落形成，而IL-3支持各种类型的单系和多系集落形成。CSF-1比IL 3支持更多的来自正常小鼠骨髓细胞的巨噬细胞集落，而在5-氟尿嘧啶处理的小鼠的骨髓细胞培养物中，IL 3支持更多的巨噬细胞集落。CSF-1和IL 3的组合导致粒细胞-巨噬细胞（GM）集落形成，其等于或大于由单独的因子支持的GM集落形成的总和。CSF-1和IL 3的组合导致巨噬细胞和嗜中性粒细胞-巨噬细胞集落的大小显著增加。在骨髓细胞与IL 3孵育5天后，向培养物中加入CSF-1，观察到集落大小的类似增加。这些数据支持这样的概念，即一些响应于IL 3的巨噬细胞集落形成细胞比那些对CSF-1敏感的细胞更原始。
Using a serum-free culture system, we examined murine macrophage colony formation from bone marrow cells cultured in the presence of purified CSF-1, interleukin 3 (IL 3) or a combination of the two factors. CSF-1 supported macrophage and neutrophil-macrophage colony formation, whereas IL-3 supported the formation of various types of single lineage and multilineage colonies. CSF-1 supported more macrophage colonies from bone marrow cells of normal mice than IL 3, whereas in cultures of bone marrow cells of 5-fluorouracil-treated mice, IL 3 supported more macrophage colonies. A combination of CSF-1 and IL 3 resulted in granulocyte-macrophage (GM) colony formation that was equal to or greater than the sum of GM colony formation supported by the factors individually. The combination of CSF-1 and IL 3 resulted in significant increases in the size of both macrophage and neutrophil-macrophage colonies. Similar increases in colony size were observed when CSF-1 was added to cultures five days after incubation of marrow cells with IL 3. These data support the concept that some of the macrophage colony-forming cells that respond to IL 3 are more primitive than those that are sensitive to CSF-1.