Protective immunity against Mycobacterium tuberculosis induced by dendritic cells pulsed with both CD8+- and CD4+-T-cell epitopes from antigen 85A
Protective immunity against Mycobacterium tuberculosis induced by dendritic cells pulsed with both CD8+- and CD4+-T-cell epitopes from antigen 85A
复制标题
DOI：
10.1128/iai.70.3.1623-1626.2002
复制
发表时间：
2002-03-01
影响因子：
3.1
通讯作者：
Hill, AVS
中科院分区：
文献类型：
--
作者：
McShane, H;Behboudi, S;Hill, AVS
在BALB/c小鼠中，先用DNA免疫，然后用表达抗原85A的修饰痘苗病毒安卡拉株免疫，诱导CD4(+)-和CD8(+)- t细胞反应。在感染结核分枝杆菌后，这种初强化方案产生的保护作用相当于牛分枝杆菌卡介苗。在用抗原85A (CD4（+)-或CD8(+)-限制性表位）脉冲的树突状细胞免疫后，单独或联合，两个表位在同一树突状细胞上的共同表达是保护所必需的，这表明诱导的CD8(+) T细胞可以发挥抵抗结核病的保护作用。
Immunization with DNA followed by modified vaccinia virus Ankara strain, both expressing the antigen 85A, induced both CD4(+)- and CD8(+)-T-cell responses in BALB/c mice. Following challenge with Mycobacterium tuberculosis, this prime-boost regimen produced protection equivalent to that conferred by Mycobacterium bovis BCG. Following immunization with dendritic cells pulsed with an antigen 85A CD4(+)- or CD8(+)-restricted epitope, alone or in combination, copresentation of both epitopes on the same dendritic cell was required for protection, demonstrating that induced CD8(+) T cells can play a protective role against tuberculosis.