Indian mustard [Brassica juncea (L.) Czern.].
Indian mustard [Brassica juncea (L.) Czern.].
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印度芥菜[Brassica juncea (L.) Czern.]。
作者：
K. Gasic;S. Korban
所有经济上重要的芸苔属物种都已成功地用根癌农杆菌转化。虽然不同的组织已被用作外植体，下胚轴仍然是最理想的外植体芸苔属植物组织培养，由于其易于再生。年轻的外植体切除3至4天的老苗表现出最佳的再生潜力，除了佐剂，如银硝酸盐的选择培养基是必要的，以实现高效率的转化。本章介绍了一种农杆菌介导的印度芥菜转化协议的基础上接种下胚轴。所用的选择标记基因编码新霉素磷酸转移酶II（npt II），选择剂为卡那霉素。
All economically important Brassica species have been successfully transformed using Agrobacterium tumefaciens. Although different tissues have been used as explants, hypocotyls remain the most desirable explants for Brassica tissue culture owing to their amenability to regeneration. Young explants excised from 3- to 4-d-old seedlings have exhibited optimal regeneration potential; the addition of adjuvants such as silver nitrate to the selection medium is necessary to achieve high efficiency of transformation. This chapter describes an Agrobacterium-mediated transformation protocol for Indian mustard based on inoculation of hypocotyls. The selectable marker gene used encodes for neomycin phosphotransferase II (nptII), and the selection agent is kanamycin.