Time-lapse observation of cell alignment on nanogrooved patterns
Time-lapse observation of cell alignment on nanogrooved patterns
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DOI：
10.1098/rsif.2008.0428.focus
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发表时间：
2009-06-06
影响因子：
3.9
通讯作者：
Iwata, Hiroo
中科院分区：
文献类型：
--
作者：
Fujita, Satoshi;Ohshima, Masahiro;Iwata, Hiroo
细胞在表面上用纳米式（NG）模式拉长，并沿该模式对齐。尽管已经提出了有关这种情况的各种模型，但仍有许多待澄清。使用固定细胞的研究并不能使自己回答其中一些开放问题。在这项研究中，使用延时显微镜观察到具有200 nm凹槽深度，870 nm脊宽度和670 nm的凹槽宽度的NG底物上活的间充质干细胞的动态行为。我们发现丝状动物像在探测细胞突起的周围环境一样移动，然后一些细胞突起入侵了探测的区域。垂直于NG方向延伸的细胞突出倾向于比平行于凹槽的细胞突出更快地缩回。从这些事实中，我们认为细胞突起的缩回阶段在沿NG模式的细胞对齐中起着规则。
Cells elongate on a surface with nanogrooved (NG) patterns and align along that pattern. Although various models have been proposed for how this occurs, much remains to be clarified. Studies with fixed cells do not lend themselves to answering some of these open questions. In this study, the dynamic behaviours of living mesenchymal stem cells on an NG substrate with a 200 nm groove depth, an 870 nm ridge width and a 670 nm groove width were observed using time-lapse microscopes. We found that filopodia moved as if they were probing the surroundings of the cell protrusion, and then some cell protrusions invaded the probed areas. Cell protrusions that extended perpendicular to the NG direction tended to retract more rapidly than those parallel to the grooves. From these facts, we think that the retracting phase of cell protrusions play a rule in cell alignment along the NG patterns.