Engineered adipose tissue supplied by functional microvessels
Engineered adipose tissue supplied by functional microvessels
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DOI：
10.1089/10763270360728170
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发表时间：
2003-12-01
影响因子：
--
通讯作者：
Stark, GB
中科院分区：
文献类型：
--
作者：
Borges, J;Mueller, MC;Stark, GB
只有通过细胞移植后的早期血管形成，才能实现体内条件下脂肪组织的体积持续化培养。自体前脂肪细胞与内皮细胞共移植可能使毛细血管网络的早期形成成为可能。体内研究是在一种特殊适应的绒毛尿囊膜(CAM)模型中进行的。受精卵在第3天孵化并打开，将人真皮微血管内皮细胞(HDMVEC)球体和前脂肪细胞在纤维蛋白基质中转移到CAM。孵育7天后，将复合材料移植，并进行免疫组织学观察。可检测到大量由HDMVEC组成的血管，这些血管的管腔内有鸡红细胞灌流。这些结果显示了由移植的HDMVEC组成的毛细血管网络的形成。鸡红细胞在由人内皮细胞组成的血管中的微循环证明了新形成的毛细血管系统与宿主血管系统的连续性。这些实验首次证明了脂肪组织中的组织工程微血管与宿主血管系统的专利连接，而无需应用外源血管生长因子或瞬时转基因。内皮细胞球体与血管生成间充质细胞的联合移植可能导致复杂的三维植入工程。
A volume-persistent culture of adipose tissue under in vivo conditions can be achieved only by early vascularization after cell transplantation. Cotransplantation of autologous preadipocytes with endothelial cells may enable the early formation of a capillary network. Investigations were performed in vivo in a specially adapted chorioallantoic membrane (CAM) model. Fertilized White Leghorn eggs were incubated and opened on day 3 of incubation and human dermal microvascular endothelial cell (HDMVEC) spheroids and preadipocytes were transferred in a fibrin matrix to the CAM. On day 7 after incubation the composites were explanted and immunohistologically investigated. Numerous vessels consisting of HDMVECs could be detected and the lumena of these vessels were perfused by chick erythrocytes. These results show the formation of a capillary network consisting of transplanted HDMVECs. The microcirculation of chick erythrocytes in vessels consisting of human endothelial cells proves the continuity of a newly formed capillary system to the host vessel system. The experiments demonstrate the first patent connection of tissue-engineered microvessels in adipose tissue to a host vessel system without applying exogenous angiogenic growth factors or transient transfection. The cotransplantation of endothelial cell spheroids with angiogenic mesenchymal cells may lead to the engineering of complex three-dimensional implants.