cDNA sequence, transport function, and genomic organization of human OCTN2, a new member of the organic cation transporter family
cDNA sequence, transport function, and genomic organization of human OCTN2, a new member of the organic cation transporter family
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DOI：
10.1006/bbrc.1998.8669
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发表时间：
1998-05-29
影响因子：
3.1
通讯作者：
Ganapathy, V
中科院分区：
文献类型：
--
作者：
Wu, X;Prasad, PD;Ganapathy, V
我们从人胎盘滋养层细胞系中克隆了OCTN 2，它是有机阳离子转运蛋白家族的一个新成员。hOCTN 2 cDNA编码具有12个推定跨膜结构域的557个氨基酸的蛋白质。octn 2基因位于人类染色体5 q31，由10个外显子组成。OCTN 2特异性转录物大小为3.5 kb，在人组织和人源细胞系中广泛表达。在氨基酸序列水平上，OCTN 2与OCTN 1的关系比与OCT 1、OCT 2和OCT 3的关系更为密切。当OCTN 2在HeLa细胞中异源表达时，它以pH依赖的方式介导了典型有机阳离子四乙基铵的转运。几种有机阳离子，包括神经毒素1-甲基-4-苯基吡啶、1-甲基-4-苯基-1，2，3，6-四氢吡啶和甲基苯丙胺，竞争OCTN 2介导的转运过程。(C)北京：科学出版社.
We have cloned OCTN2, a new member of the organic cation transporter family, from a human placental trophoblast cell line. The hOCTN2 cDNA codes for a protein of 557 amino acids with twelve putative transmembrane domains. The octn2 gene, located on human chromosome 5q31, consists of ten exons. The OCTN2-specific transcript, 3.5 kb in size, is expressed widely in human tissues and in cell lines of human origin. At the level of amino acid sequence, OCTN2 is more closely related to OCTN1 than to OCT1, OCT2 and OCT3, When expressed heterologously in HeLa cells, OCTN2 mediates the transport of tetraethylammonium, a prototypical organic cation, in a pH-dependent manner. Several organic cations, including the neurotoxins 1-methyl-4-phenylpyridinium, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, and methamphetamine, compete for the OCTN2-mediated transport process. (C) 1998 Academic Press.