Addressable discrete-line-scanning multiphoton microscopy based on a spatial light modulator.
Addressable discrete-line-scanning multiphoton microscopy based on a spatial light modulator.
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DOI：
10.1364/ol.37.000827
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发表时间：
2012-03-01
期刊：
影响因子：
3.6
通讯作者：
Gao B
中科院分区：
文献类型：
--
作者：
Qin W;Shao Y;Liu H;Peng X;Niu H;Gao B
我们开发了一种基于空间光调制器的新型可寻址离散线扫描多光子显微镜，具有高横向和轴向分辨率。我们的离散线聚焦设计消除了传统一维线扫描多光子显微镜中出现的串扰。此外，仅相位空间光调制器能够根据目标区域的形状和位置生成特定的离散线焦点，从而仅扫描样本的目标区域。与其他多光子显微镜相比，该技术通过将扫描能量和停留时间集中在感兴趣的区域，缩短了扫描时间并最大限度地减少了光损伤。
We developed a novel addressable discrete-line-scanning multiphoton microscope with high lateral and axial resolutions based on a spatial light modulator. Our discrete-line-focus design eliminates the cross talk that occurs in conventional one-dimensional line-scanning multiphoton microscopies. Additionally, a phase-only spatial light modulator is able to scan only a sample’s target area by generating a specific discrete line focus according to the shape and location of the target area. Compared with other multiphoton microscopies, this technique shortens scanning time and minimizes photodamage by concentrating scanning energy and dwell time on the area of interest.