Identification of Follicular T Helper Cells in Tissue Sections
Identification of Follicular T Helper Cells in Tissue Sections
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DOI：
10.1007/978-1-4939-2498-1_2
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Hsu, Hui-Chen
中科院分区：
文献类型：
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作者：
Ding, Yanna;Mountz, John D.;Hsu, Hui-Chen
滤泡性T辅助细胞（Tfh）是主要位于生殖中心（GC）以帮助B细胞成熟和抗体产生的CD 4 T辅助细胞的关键群体。Tfh细胞可以基于一组经典Tfh表面标记物、转录标记物和效应子功能细胞因子的表达，以及通过其与其他GC细胞（包括滤泡树突细胞（FDC）和GC B细胞）的独特解剖学接近性在组织切片中鉴定。在这里，我们描述了一种免疫荧光染色方法的可视化GC Tfh细胞冷冻脾组织切片的自身免疫BXD 2小鼠使用共聚焦成像策略。基于⑶ 4、CXCR 5、Bcl 6、IL-21和IL-17的表达表征Tfh细胞。
Follicular T helper (Tfh) cells are a critical population of CD4 T helper cells that are primarily localized in the germinal centers (GCs) to help B cell maturation and antibody production. Tfh cells can be identified in tissue sections based on the expression of a panel of classical Tfh surface makers, transcription marker(s), and effector-function cytokines, as well as by their unique anatomic proximity to other GC cells, including follicular dendritic cells (FDC) and GC B cells. Here, we describe an immunofluorescence staining method for visualization of GC Tfh cells in frozen spleen tissue sections of the autoimmune BXD2 mouse using a confocal imaging strategy. Tfh cells were characterized based on the expression of CD4, CXCR5, Bcl6, IL-21, and IL-17.