Size-exclusion SPR sensor chip: application to detection of aggregation and disaggregation of biological particles
Size-exclusion SPR sensor chip: application to detection of aggregation and disaggregation of biological particles
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DOI：
10.1039/c2an16010b
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Oohira, Fumikazu
中科院分区：
文献类型：
--
作者：
Terao, Kyohei;Shimizu, Kazunori;Oohira, Fumikazu
提出了一种新型的表面等离子体共振（SPR）传感器芯片，该芯片采用微细缝阵列。微狭缝将大于其狭缝尺寸的微米级物体排除在SPR传感区域之外，从而起到原位滤波器的作用。我们演示了用该芯片对不同直径的微粒子的传感，结果显示了成功的尺寸排除效应。作为生物应用的演示，我们以糖链固定的金纳米颗粒为测试样本，对生物颗粒的聚集和分解进行了检测。
We propose a novel surface plasmon resonance (SPR) sensor chip with a microfabricated slit array. The microslit excludes micrometre-size objects larger than its slit size from the SPR sensing area, so that it functions as an in situ filter. We demonstrated the sensing of microparticles of different diameters using the chip, and the results show a successful size-exclusion effect. As a demonstration of the biological application, we performed the detection of aggregation and disaggregation of biological particles using sugar-chain-immobilized gold nanoparticles as a test sample.