TRANSPORT OF SUGARS IN TUMOR-CELL MEMBRANES
TRANSPORT OF SUGARS IN TUMOR-CELL MEMBRANES
复制标题
DOI：
10.1016/0304-419x(74)90008-0
复制
发表时间：
1974-01-01
期刊：
影响因子：
--
通讯作者：
HATANAKA, M
中科院分区：
文献类型：
--
作者：
HATANAKA, M
Please try later.
Are there any differences between normal and tumor cell membranes in the transport of sugars? This is the question we are asking and our answer is yes, as detailed in this review. Warburg reported many years ago that tumor cells are more glycolytic than normal cells, particularly under aerobic conditions [1]. Although he hypothesized that this higher rate of aerobic glycolysis in tumors results from an impairment in respiration [2], there has been some debate against this hypothesis, claiming that growth rather than malignancy is the primary cause of the high rate of glycolysis [3-5]. However, more recently, Rubin's group studied the influence of cell density on the rate of glycolysis and concluded that population density per se, and not growth rate, appeared to be responsible for the changes in the pattern of carbon flow [6].1 suggested that sugar transport should be considered as one part of cellular metabolism and thus the very first step of a rate-limiting reaction for the overall metabolism of sugar in cells including catabolic and anabolic pathways. For instance, glucose, known as the most efficient natural catabolite source, may be transported differently depending on various cell conditions. Previous to this suggestion, there was no report showing that sugar transport per se was changed by transformation and no attempt to measure sugar transport as distinct from overall sugar metabolism. Therefore, I decided to investigate the possibility of a change in transport of sugars coincident with transformation. This became possible because of the ability to transform normal cells in tissue culture by tumor virus infection, where experimental conditions could be controlled at will. It was not possible to show any difference in sugar uptake after SV4o or polyoma virus (DNA tumor virus) infection, probably because the rate of transformation was low in the non-permissive but transformable host cells. About that time, a very efficient transformation of mammalian cells by murine sarcoma virus was described [7, 8] and further technical developments involved the use of chicken cells, which were 100~ A transformed in 20 h after infection by Rous sarcoma virus (RSV)[9]. The initial aim to study change of sugar transport by transformation was helped enormously by the timely discoveries of these sarcoma viruses and attendant technical developments. In this review, the word" transport" is used when presumably only a plasma membrane function is involved, while" uptake" is used when intracellular, as well as membrane functions, may be involved.