The worm has turned: Behavioural drivers of reproductive isolation between cryptic lineages
The worm has turned: Behavioural drivers of reproductive isolation between cryptic lineages
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DOI：
10.1016/j.soilbio.2016.03.015
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发表时间：
2016-07-01
影响因子：
9.7
通讯作者：
Novo, M.
中科院分区：
文献类型：
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作者：
Jones, G. L.;Wills, A.;Novo, M.
物种识别和配偶吸引信号等行为过程加强并巩固了物种形成所需的生殖隔离。英国的红正蚓（Lumbricus rubellus）深度分化为两个主要的遗传谱系“A”和“B”。在某些地点，它们常常同域分布，但不清楚它们在某种程度上是否存在生殖隔离。进行了行为测试，其中对单独进行基因分型的蚯蚓能够在之前由基因相似或不相似个体翻动过的土壤之间进行选择（N = 45）。我们发现，个体（75%）明显（P < 0.05）更有可能朝着由自身谱系的蚯蚓翻动过的土壤定向。进一步的测试涉及一种基于滤纸的选择设计，滤纸两侧分别用由不同基因型蚯蚓翻动过的土壤的水提取物浸湿（N = 18），或者用翻动过的土壤提取物与未翻动的对照土壤提取物进行对比（N = 10）。再次，蚯蚓朝着来自其同类基因型的提取物定向（P < 0.05）。这些发现表明，红正蚓会释放基因型特异性的水溶性化学物质；此外，它们会改变行为，并通过交配前的选型交配在隐性姊妹种的同域蚯蚓谱系之间的生殖隔离中发挥作用。（C）2016作者。由爱思唯尔有限公司出版。
Behavioural processes such as species recognition and mate attraction signals enforce and reinforce the reproductive isolation required for speciation. The earthworm Lumbricus rubellus in the UK is deeply differentiated into two major genetic lineages, 'A' and 'B'. These are often sympatric at certain sites, but it is not known whether they are to some extent reproductively isolated. Behavioural tests were performed, in which individually genotyped worms were able to choose between soils previously worked either by genetically similar or dissimilar individuals (N = 45). We found that individuals (75%) were significantly (P < 0.05) more likely to orientate towards the soil conditioned by worms of their own lineage. Further testing involved a choice design based on filter papers wetted with water extracts of soils worked by a different genotype on each side (N = 18) or extracts from worked soil vs. un-worked control soil (N = 10). Again, earthworms orientated towards the extract from their kindred genotype (P < 0.05). These findings indicate that genotype-specific water-soluble chemicals are released by L. rubellus; furthermore, they are behaviour-modifying, and play a role in reproductive isolation between sympatric earthworm lineages of cryptic sibling species, through pre-copulatory assortative mate choice. (C) 2016 The Authors. Published by Elsevier Ltd.