Staphylococcus epidermidis undergoes global changes in gene expression during biofilm maturation in platelet concentrates
Staphylococcus epidermidis undergoes global changes in gene expression during biofilm maturation in platelet concentrates
复制标题
DOI：
10.1111/trf.16418
复制
发表时间：
2021-05-17
期刊：
影响因子：
2.9
通讯作者：
Ramirez-Arcos, Sandra
中科院分区：
文献类型：
--
作者：
Loza-Correa, Maria;Yousuf, Basit;Ramirez-Arcos, Sandra
背景：表皮葡萄球菌在血小板浓缩物（PCs）中生长时形成表面附着的聚集体（生物膜）。采用比较转录组分析方法研究了表皮葡萄球菌生物膜在pc中的差异基因表达。研究设计与方法：将2株从人皮肤中分离的表皮葡萄球菌（AZ22和AZ39）和1株从污染pc中分离的表皮葡萄球菌（ST02）分别培养在葡萄糖添加胰酶豆汤（TSBg）和pc中。提取RNA并使用Illumina HiSeq进行测序。采用DESeq进行差异表达分析，筛选出显著差异表达基因（DEGs）。对基因和基因组进行京都百科全书和基因本体分析。用定量反转录pcr验证差异基因表达。结果：AZ22、AZ39和ST02分别表达了436、442和384个基因。DEG分析显示，与TSBg相比，pc中有170、172和117个基因上调，而在AZ22、AZ39和ST02成熟生物膜中分别有120、135和89个基因下调（p < 0.05）。这三个菌株共有27个deg。AZ22和AZ39共有76度，而ST02和AZ22、AZ39共有34度和21度。在参与血小板-细菌相互作用、生物膜形成、毒力因子的产生以及对抗菌肽和抗生素的耐药性的基因中观察到显著的转录表达变化。结论：表皮葡萄球菌基因的差异表达是由应激贮藏环境引起的。毒力和抗菌素耐药基因的上调可能对输血患者具有临床意义。
Background: Staphylococcus epidermidis forms surface-attached aggregates (biofilms) when grown in platelet concentrates (PCs). Comparative transcriptome analyses were undertaken to investigate differential gene expression of S. epidermidis biofilms grown in PCs.Study design and methods: Two S. epidermidis strains isolated from human skin (AZ22 and AZ39) and one strain isolated from contaminated PCs (ST02) were grown in glucose-supplemented Trypticase Soy Broth (TSBg) and PCs. RNA was extracted and sequenced using Illumina HiSeq. Differential expression analysis was done using DESeq, and significantly differentially expressed genes (DEGs) were selected. DEGs were subjected to Kyoto encyclopedia of genes and genomes and Gene Ontology analyses. Differential gene expression was validated with quantitative reverse transcription-PCR.Results: A total of 436, 442, and 384 genes were expressed in AZ22, AZ39, and ST02, respectively. DEG analysis showed that 170, 172, and 117 genes were upregulated in PCs in comparison to TSBg, whereas 120, 135, and 89 genes were downregulated (p < .05) in mature biofilms of AZ22, AZ39, and ST02, respectively. Twenty-seven DEGs were shared by all three strains. While 76 DEGs were shared by AZ22 and AZ39, only 34 and 21 DEGs were common between ST02, and AZ22 and AZ39, respectively. Significant transcriptional expression changes were observed in genes involved in platelet-bacteria interaction, biofilm formation, production of virulence factors, and resistance to antimicrobial peptides and antibiotics.Conclusion: Differential gene expression in S. epidermidis is triggered by the stressful PC storage environment. Upregulation of virulence and antimicrobial resistance genes could have clinical implications for transfusion patients.