Circular compartmentalized microfluidic platform: Study of axon-glia interactions
Circular compartmentalized microfluidic platform: Study of axon-glia interactions
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DOI：
10.1039/b918640a
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Venkatesan, Arun
中科院分区：
文献类型：
--
作者：
Hosmane, Suneil;Yang, In Hong;Venkatesan, Arun
我们描述了一个区隔化的圆形微流体平台，可以在定义的微环境中定向放置细胞，以研究轴突-胶质细胞的相互作用。多室平台由径向微通道阵列的独立单元组成，可从轴突室中流体分离出染色体。在微通道附近穿孔的流体访问端口允许将细胞直接移液到设备中。相邻的小体或轴突室室可以很容易地合并，因此独立的神经元群或轴突群可以维持在分离或统一的微环境中。我们在该装置中展示了三种不同的定向细胞放置模式，以适应轴突-胶质细胞相互作用研究的不同实验需求：(1)圆形平台的离心可导致微通道中轴突通量增加两倍，并提供了一种建立轴突-胶质细胞相互作用的新技术；(2)微模板可用于直接将胶质细胞放置在感兴趣的区域内；(3)通过标准移液管技术可以实现轴突-胶质细胞的亲密共培养。我们利用这种微流控平台来证明小胶质细胞在受损中枢神经系统轴突附近的双重优先积累，这一事件涉及许多慢性神经炎症和神经退行性疾病的维持和进展。
We describe a compartmentalized circular microfluidic platform that enables directed cell placement within defined microenvironments for the study of axon-glia interactions. The multi-compartment platform consists of independent units of radial microchannel arrays that fluidically isolate somal from axonal compartments. Fluidic access ports punched near the microchannels allow for direct pipetting of cells into the device. Adjacent somal or axonal compartments can be readily merged so that independent groups of neurons or axons can be maintained in either separate or uniform microenvironments. We demonstrate three distinct modes of directed cell placement in this device, to suit varying experimental needs for the study of axon-glia interactions: (1) centrifugation of the circular platform can result in a two-fold increase in axonal throughput in microchannels and provides a new technique to establish axon-glia interactions; (2) microstencils can be utilized to directly place glial cells within areas of interest; and (3) intimate axon-glia co-culture can be attained via standard pipetting techniques. We take advantage of this microfluidic platform to demonstrate a two-fold preferential accumulation of microglia specifically near injured CNS axons, an event implicated in the maintenance and progression of a number of chronic neuroinflammatory and neurodegenerative diseases.