DNA COMPARISONS OF TRYPANOSOMA-EVANSI (INDONESIA) AND TRYPANOSOMA-BRUCEI SPP
DNA COMPARISONS OF TRYPANOSOMA-EVANSI (INDONESIA) AND TRYPANOSOMA-BRUCEI SPP
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DOI：
10.1017/s0031182000060819
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发表时间：
1992-02-01
期刊：
影响因子：
2.4
通讯作者：
DONELSON, JE
中科院分区：
文献类型：
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作者：
ARTAMA, WT;AGEY, MW;DONELSON, JE
通过聚合酶链反应（PCR）分析发现，来自印度尼西亚伊氏锥虫分离株的两个克隆含有3种不同的布氏锥虫核DNA重复序列：高度重复的177个碱基对的共有序列和编码前环素和剪接前导序列的基因重复序列。 此外，对其中一个克隆的994 bp小环序列进行了测定，并对T. evansi中不扩增T.布氏杆菌克隆测试
Two clones from separate isolates of Trypanosoma evansi in Indonesia were found by polymerase chain reaction (PCR) analyses to contain 3 different repeated nuclear DNA sequences of Trypanosoma brucei spp: the consensus sequence for a highly repetitive 177 base pairs and the gene repeats encoding procyclin and the spliced leader. In addition, the 994 bp minicircle sequence of one of the clones was determined, and PCR amplification primers specific for minicircles of T. evansi were identified that do not amplify minicircle sequences in the T. brucei spp. clones tested.