Engineered topographical structure to control spatial cell density using cell migration
Engineered topographical structure to control spatial cell density using cell migration
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DOI：
10.1007/s10544-019-0447-0
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发表时间：
2019-11
影响因子：
2.8
通讯作者：
C. Okutani;A. Wagatsuma;K. Mabuchi;T. Hoshino
中科院分区：
文献类型：
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作者：
C. Okutani;A. Wagatsuma;K. Mabuchi;T. Hoshino
构建功能性组织需要控制各种细胞类型的空间分布。在这里，我们报告了一个简单的地形结构改变了空间细胞密度。设计了一个凹形的弯曲边界，允许细胞的空间下降运动和共培养细胞的空间分布的变化。我们利用成肌细胞（C2C12）和神经元细胞（PC12）之间的细胞运动性的差异来证明空间细胞密度自发变化的可行性。在没有弯曲边界的情况下，高运动性细胞（C2C12）不会迁移到相邻区域，这导致空间细胞分布的轻微时间变化（< 15%）。相反，在弯曲边界的情况下，沟槽中的高运动性细胞的细胞密度相对于脊上的那些细胞显示出超过45%的增加。另一方面，低运动细胞（PC12）的空间细胞分布的时间变化低于15%，有或没有弯曲的边界。此外，随着沟宽的增加，两种细胞在沟内的聚集程度增加。重要的是，这些细胞类型依赖性结果也在共培养条件下得以维持。我们的研究结果表明，设计拓扑接口改变空间细胞密度没有任何操作，是有用的多细胞结构。
Control of the spatial distribution of various cell types is required to construct functional tissues. Here, we report a simple topographical structure changed the spatial cell density. A concave curved boundary was designed, which allowed the spatial descent moving of cells and the change in spatial distributions of co-cultured cells. We utilized the difference in cell motility between myoblast cells (C2C12) and neuronal cells (PC12) to demonstrate the feasibility of spontaneous change in spatial cell density. Without the curved boundaries, high motility cells (C2C12) did not migrate to the adjacent area, which resulted in a slight temporal change (< 15%) in the spatial cell distribution. In contrast, with the curved boundaries, the cell density of the high motility cells in the groove to those cells on the ridge showed an increase exceeding 45%. On the other hand, the temporal change in the spatial cell distribution of low motility cells (PC12) was below 15% with or without the curved boundaries. In addition, as groove width increased, both cells displayed more initially gathering in groove. Importantly, these cell-type dependent results were also maintained under co-culture conditions. Our results suggest that designing topographical interfaces changes spatial cell density without any manipulation and is useful for multi-cellular constructs.