Phenotype analysis by MUC2, MUC5AC, MUC6, and CD10 expression in Epstein-Barr virus-associated gastric carcinoma
Phenotype analysis by MUC2, MUC5AC, MUC6, and CD10 expression in Epstein-Barr virus-associated gastric carcinoma
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DOI：
10.1007/s00535-006-1841-y
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发表时间：
2006-09-01
影响因子：
6.3
通讯作者：
Fukayama, Masashi
中科院分区：
文献类型：
--
作者：
Barua, Rita Rani;Uozaki, Hiroshi;Fukayama, Masashi
后面。胃粘膜标志物粘蛋白（MUC 5AC和MUC 6）和肠粘膜标志物粘蛋白（MUC 2和CD 10）已被用于胃癌上皮细胞的细胞谱系鉴定。方法.为明确EB病毒（EBV）相关性胃癌的特点，对18例EBV相关性胃癌和56例EBV阴性胃癌进行了沿着化学分析。结果EBV相关性胃癌中MUC 2表达较低：免疫染色0级、1级、2级、3级和4级在EBV相关性胃癌中分别为10例、6例、1例、1例和0例，在EBV阴性胃癌中分别为18例、11例、15例、6例和6例（P = 0.013）。CD 10阳性率（2-4级）在EBV相关性胃癌中为6%，显著低于EBV阴性胃癌（34%）（P = 0.030）。当通过胃标志物（MUC 5AC或MUC 6）和肠标志物（MUC 2或CD 10）的联合阳性对GC的表型进行分类时，EBV相关GC主要包括无效（44%）和胃（39%）型，但EBV阴性GC包括无效（7%）、胃（30%）、肠（27%）和混合（36%）型。EBV相关性胃癌和EBV阴性胃癌患者的年龄明显年轻。结论. EB病毒相关性胃癌的肿瘤上皮细胞不表达MUC 2或CD 10，大多数被归类为空型或胃型。EBV感染可能发生在无效或胃表型的上皮细胞中，其可能缺乏向肠表型的转分化潜力。
Backround. Gastric marker mucins (MUC5AC and MUC6) and intestinal marker molecules (MUC2 and CD10) have been used to determine the cell lineage of epithelial cell of gastric carcinoma (GC). Methods. To clarify the characteristics of Epstein-Barr virus (EBV)-associated GC, 18 cases were immunohistochemically evaluated along with 56 cases of EBV-negative GC. Results. MUC2 expression was lower in EBV-associated GC: immunostaining grades 0, 1, 2, 3, and 4 were observed in 10, 6, 1, 1, and 0 cases of EBV-associated GC, respectively, and in 18, 11, 15, 6, and 6 cases of EBV-negative GC, respectively (P = 0.013). CD10 positivity (grades 2-4) in EBV-associated GC was 6%, significantly lower than in EBV-negative GC (34%) (P = 0.030). When phenotypes of GC were categorized by the combined positivities of gastric markers (either MUC5AC or MUC6) and intestinal markers (either MUC2 or CD10), EBV-associated GC included primarily null (44%) and gastric (39%) types, but EBV-negative GC comprised null (7%), gastric (30%), intestinal (27%), and mixed (36%) types. The age of patients with gastric types was significantly younger for both EBV-associated GC and EBV-negative GC cases. Conclusions. Neoplastic epithelial cells of EBV-associated GC did not express MUC2 or CD10, and most of them were categorized as null or gastric types. EBV infection may occur in the epithelial cells of null or gastric phenotypes, which may be devoid of transdifferentiation potential toward intestinal phenotypes.