Persistence of Parvovirus B19 (B19V) DNA and humoral immune response in B19V-infected blood donors
Persistence of Parvovirus B19 (B19V) DNA and humoral immune response in B19V-infected blood donors
复制标题
DOI：
10.1111/vox.12162
复制
发表时间：
2014-10-01
期刊：
影响因子：
2.7
通讯作者：
Hennig, H.
中科院分区：
文献类型：
--
作者：
Juhl, D.;Goerg, S.;Hennig, H.
背景和目的 细小病毒 B19 (B19V) DNA 似乎持续存在于 B19V 感染的献血者的血浆中。这与单一献血者血液成分的接受者的相关性尚不清楚。材料和方法我们研究了 75 名 B19V 感染献血者的连续档案和随访样本，以获得有关病毒血症的持续时间和程度以及抗 B19V IgG 和 IgM 存在的更多数据。通过蛋白质印迹分析对 29 名供体中的 IgG 抗体进行了进一步表征。结果 在收集的 411 份 B19V DNA 阳性样本中，我们发现其中 5 份样本浓度较高（>10(6) IU B19V DNA/ml 血浆）。所有捐赠者的 B19V DNA 平均持续时间为 21.5 个月（范围：2.3-52.4；95% 置信区间，19.1-23.9 个月）。只有 15 个此类样本不含或低滴度 IgG 抗 B19V。 IgG 抗体主要针对次要衣壳蛋白 VP1 上的表位，因此可能是具有高亲合力的中和型抗体。在 9/13 具有高 DNA 浓度的样本中可检测到 IgM 抗 B19V。 结论 绝大多数具有可检测到的 B19V DNA 的单一供体血液成分对其接受者来说可能不具有感染性，因为 DNA 水平较低，而且供体也具有高亲合力的潜在中和抗体。抗 B19V IgM 测试并不能识别每一次具有高 B19V DNA 浓度的献血，但是，除了 B19V NAT 测试之外，具有持续 IgG 抗 B19V 的献血者可能被认为对于单一献血者血液成分来说是“B19V 安全”的。
Background and Objectives Parvovirus B19 (B19V) DNA seems to persist in the plasma of B19V-infected blood donors. The relevance of this for recipients of single-donor blood components is yet unclear.Material and Methods We studied serial archive and follow-up samples from 75 B19V-infected blood donors to obtain more data about the duration and degree of viraemia and the presence of IgG and IgM anti-B19V. IgG antibodies were further characterized by Western blot analysis in 29 donors.Results In 411 B19V DNA-positive samples collected, we found high concentrations (>10(6) IU B19V DNA/ml plasma) in five. B19V DNA persisted for a mean of 21.5 months (range: 2.3-52.4; 95% confidence interval, 19.1-23.9 months) in all donors. Only 15 such samples had either no or low-titre IgG anti-B19V. IgG antibodies were predominantly directed against epitopes on the minor capsid protein VP1, thus probably of neutralizing type with high avidity. IgM anti-B19V was detectable in 9/13 samples with high DNA concentrations.Conclusions The vast majority of single-donor blood components with detectable B19V DNA are probably not infectious for their recipients because DNA is at only low levels and the donors also have potentially neutralizing antibodies with high avidity. Anti-B19V IgM testing does not identify every donation with high B19V DNA concentrations, but, in addition to B19V NAT testing, donors with persistent IgG anti-B19V might be considered 'B19V-safe' for single-donor blood components.