The teleost Oryzias latipes shows telomere shortening with age despite considerable telomerase activity throughout life
The teleost Oryzias latipes shows telomere shortening with age despite considerable telomerase activity throughout life
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DOI：
10.1016/j.mad.2008.05.006
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发表时间：
2008-09-01
影响因子：
5.3
通讯作者：
Ishikawa, Naoshi
中科院分区：
文献类型：
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作者：
Hatakeyama, Hitoshi;Nakamura, Ken-Ichi;Ishikawa, Naoshi
以前对端粒和端粒酶的研究主要集中在哺乳动物身上，其他脊椎动物的数据有限。我们分析了端粒长度（末端限制性片段长度）和端粒酶活性在一个小的淡水硬骨鱼，meclaka（Oryzias latipes），并发现，端粒缩短衰老过程中，尽管事实上，相当数量的端粒酶活性是无处不在的整个生命的鱼检测。由于端粒的磨损率在发育过程中大于成年，端粒长度与体长的增加呈负相关。青鳉个体间端粒长度的差异与人类相似，个体特异性差异甚至在最早的胚胎阶段就很明显。端粒酶活性不仅在胚胎体内，而且在成熟个体的整个生命周期中的全身器官中普遍可检测到。这些数据表明，端粒磨损老化过程中的meclaka，这是类似于在人类中，可能是一个主要因素，决定他们的死亡率，并通过强大的端粒酶活性端粒维护可能需要的特征终身连续增长的这条鱼。(c)2008爱思唯尔爱尔兰有限公司保留所有权利。
Previous studies of telomeres and telomerase have focused mostly on mammals, and data for other vertebrates are limited. We analyzed both telomere length (terminal restriction fragment length) and telomerase activity in a small freshwater teleost fish, the meclaka (Oryzias latipes), and found that the telomeres shorten during ageing despite the fact that a considerable amount of telomerase activity is ubiquitously detectable throughout the life of the fish. Since the telomere attrition rate during development was greater than that in adulthood, telomere length is inversely correlated with the increase in body length. The difference in telomere length among medaka individuals was similar to that in humans, and the individual specific differences were evident even at the earliest embryonic stage. Telomerase activity was ubiquitously detectable not only in the body of the embryo but also in the systemic organs of mature individuals throughout their entire life span. These data suggest that telomere attrition during ageing in meclaka, which is similar to that in humans, may be a major factor determining their mortality, and that telomere maintenance through strong telomerase activity may be required for the characteristic lifelong continuous growth of this fish. (c) 2008 Elsevier Ireland Ltd. All rights reserved.