Simultaneous banding of rat embryo DNA, RNA, and protein in cesium trifluoroacetate gradients.
Simultaneous banding of rat embryo DNA, RNA, and protein in cesium trifluoroacetate gradients.
复制标题
在三氟乙酸铯梯度中同时显带大鼠胚胎 DNA、RNA 和蛋白质。
DOI：
10.1016/0003-2697(85)90242-8
复制
发表时间：
1985
影响因子：
2.9
通讯作者：
Mirkes,PE
中科院分区：
文献类型：
--
作者：
Mirkes,PE
描述了一种在三氟乙酸铯(CsTFA)梯度中通过离心法同时显带细胞DNA、RNA和蛋白质的方法。从第11天大鼠胚胎的匀浆开始，这个程序被用来分离总DNA、RNA和蛋白质。在DNA、RNA和蛋白质的峰密度分别为1.63g/ml、1.83g/ml和1.40g/ml的条件下，从CsTFA梯度中提取的核酸是不含蛋白质的。用这种方法提取的RNA显然没有DNA污染，但用这种方法提取的DNA确实含有一定的RNA(低于5%的污染)。
A procedure for the simultaneous banding of cellular DNA, RNA, and protein by centrifugation in cesium trifluoracetate (CsTFA) gradients is described. Starting with homogenates of Day 11 rat embryos, this procedure was used to separate total DNA, RNA, and protein. Under the conditions used DNA banded at a peak density of 1.63 g/ml, RNA at a peak density of 1.83 g/ml, and protein at a peak density of 1.40 g/ml. Nucleic acids isolated from CsTFA gradients were judged to be protein free. RNA isolated by this method is apparently free of DNA contamination; however, DNA isolated by this method does contain some RNA (less than 5% contamination).