Gonadal hormones act extrinsic to the hippocampus to influence the density of hippocampal astroglial processes.
Gonadal hormones act extrinsic to the hippocampus to influence the density of hippocampal astroglial processes.
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性腺激素作用于海马外来影响海马星形胶质细胞突起的密度。
DOI：
10.1016/s0306-4522(02)00730-3
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发表时间：
2003
期刊：
影响因子：
3.3
通讯作者：
Leranth,C
中科院分区：
文献类型：
--
作者：
Lâm,TT;Leranth,C
雌激素对海马神经元形态学的重要影响已得到充分证实。然而，导致这种变化的机制已经被证明是令人困惑的复杂，因为胶质细胞和神经元之间的相互作用，以及来自其他脑区的神经元影响，都涉及其中。这项研究解决了雌激素敏感的预测从内侧隔/斜角带的布罗卡诱导星形胶质细胞反应的可能性。将雌激素和胆固醇填充（对照）插管植入成年卵巢切除大鼠的Broca内侧隔/斜角带。比较半定量免疫组织化学分析的密度的胶质细胞的酸性蛋白的过程和细胞进行了局部雌激素治疗和对照动物的海马切片。接受雌激素填充插管的大鼠海马CA 1和CA 3子区的胶质细胞突起密度低于对照组动物。在齿状回中未观察到这些效应。细胞计数显示，在任何检查区域的胶质细胞酸性蛋白阳性细胞的数量没有显着差异。从这些结果中可以得出两个主要结论。首先，数据表明，雌激素，事实上，通过隔-海马投射对海马细胞有间接影响。此外，雌二醇可以对海马星形胶质细胞产生间接的负面影响，导致其突起密度降低。
The important effects of estrogen on the morphology of hippocampal neurons are well established. The mechanisms leading to such changes, nevertheless, have proved confusingly complex, since interactions between glia and neurons, as well as neuronal influences from other brain fields, are involved. This study addresses the possibility that estrogen-sensitive projections from the medial septum/diagonal band of Broca induce astroglial reactions. Estrogen- and cholesterol-filled (controls) cannulae were implanted into the medial septum/diagonal band of Broca of adult ovariectomized rats. Comparative semiquantitative immunohistochemical analysis on the density of the glial fibrillary acidic protein-containing processes and cells were performed on hippocampal slices of locally estrogen-treated and control animals. Rats that received estrogen-filled cannulae showed a lower density of glial processes in the hippocampal CA1 and CA3 subfields than animals of the control group. These effects could not be observed in the dentate gyrus. Cell counts revealed no significant difference in the number of glial fibrillary acidic protein-positive cells in any of the examined areas. Two major conclusions can be drawn from these results. First, the data show that estrogen, in fact, has an indirect influence on hippocampal cells through septo-hippocampal projections. Furthermore, estradiol can have an indirect negative effect on hippocampal astrocytes, causing a reduction in the density of their processes.