Placental mitochondrial DNA content and placental abruption: a pilot study.
Placental mitochondrial DNA content and placental abruption: a pilot study.
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DOI：
10.1186/s13104-015-1340-4
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发表时间：
2015-09-16
影响因子：
1.8
通讯作者：
Williams, Michelle A
中科院分区：
文献类型：
--
作者：
Qiu, Chunfang;Sanchez, Sixto E;Williams, Michelle A
背景：线粒体的生物发生和充足的能量生产对于胚胎发生和胎盘形成是重要的。先前的研究记录了母体血液线粒体DNA（mtDNA）拷贝数的改变-线粒体功能障碍的标志-在妊娠并发胎盘早剥中。为了进一步了解线粒体功能障碍在胎盘脱垂发病机制中的作用，我们对103例胎盘脱垂病例和102例非胎盘脱垂对照的胎盘标本进行了初步研究。实时定量聚合酶链反应（PCR）用于评估从分娩后立即收集的胎盘样本中提取的DNA中mtDNA的相对拷贝数。结果：胎盘早剥的危险性随mtDNA拷贝数的增加而增加（p = 0.05），胎盘早剥的危险性随mtDNA拷贝数的增加而增加（p = 0.05）。胎盘线粒体DNA拷贝数较高（≥120.5，中位数）的孕妇胎盘早剥的几率高于线粒体DNA拷贝数较低（
BACKGROUND: Mitochondrial biogenesis and adequate energy production are important for embryogenesis and placentation. Previous studies documented alterations in maternal blood mitochondrial DNA (mtDNA) copy number-a marker of mitochondrial dysfunction-in pregnancies complicated by placental abruption. To further understand the role of mitochondrial dysfunction in the pathogenesis of placental abruption, we conducted a pilot study using placental specimen collected from 103 placental abruption cases and 102 non-abruption controls. Real-time quantitative polymerase chain reaction (PCR) was used to assess the relative copy number of mtDNA in DNA extracted from placental samples collected immediately after delivery. Logistic regression procedures were used to estimate adjusted odds ratios (OR) and 95% confidence intervals (CI).RESULTS: Higher odds of placental abruption was observed with increasing mtDNA copy number (p value for trend = 0.05). The odds of placental abruption was elevated among women who delivered placentas with higher mtDNA copy number (≥120.5, the median) as compared with those with lower values (