Influence of bacterial, environmental and physical factors in design of photocatalytic reactors for water disinfection
Influence of bacterial, environmental and physical factors in design of photocatalytic reactors for water disinfection
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DOI：
10.1016/j.jphotochem.2018.04.030
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发表时间：
2018-11-01
影响因子：
4.3
通讯作者：
Robertson, Jeanette M. C.
中科院分区：
文献类型：
--
作者：
Buck, Caitlin;Skillen, Nathan;Robertson, Jeanette M. C.
用于细菌消毒研究的光催化反应器的设计通常基于定义的实验室条件，这些条件并不能真实地代表细菌在自然界中遇到的恶劣和不断变化的环境。在这项研究中，四个参数（生长阶段，生物膜生产，pH值和照射源）subject.to连续通量的性质，。对影响光催化消毒效果的主要因素进行了分析，并指出了它们在工艺设计中的重要性。研究结果产生了一些关键的发现，在设计用于生物过程的光催化反应器时应该考虑到这些发现。使用大肠杆菌作为模式生物，pH值和细菌生长阶段的影响的研究表明，在稳定期和pH值为8的细胞更耐光催化分解。只有在pH值为4，而在对数生长期，是完全实现光催化破坏。通过将六个8 W的黑灯替换为以1.05 W工作的单个高功率UV-LED，进一步增强了该过程。通过比较表皮葡萄球菌的生物膜产生和非产生菌株的光催化破坏来研究毒力的影响。结果表明，消毒的敏感性没有差异，这表明单独表达毒力因子的能力可能不会产生更大的抵抗光催化破坏。
The design of photocatalytic reactors for use in bacterial disinfection studies is often based on defined laboratory conditions, which are not a true representation of the harsh and ever-changing environment that bacteria encounter in nature. In this study four parameters (growth phase, biofilm production, pH and irradiation source) subject.to continuous flux in nature,. which could affect the efficacy of photocatalytic disinfection stndies were examined and their importance in process design was considered. The results produced a number of key findings which should be taken into consideration when designing photocatalytic reactors for biological processes. Using Escherichia coli as a model organism, studies of effects of pH and bacterial growth phase showed that cells in the stationary phase and at a pH of 8 were more resistant to photocatalytic breakdown. Only at a pH of 4 and while in the logarithmic growth phase, was complete photocatalytic destruction achieved. This process was further enhanced by replacing six 8 W black lamps with a single high-power UV-LED operated at 1.05 W. The impact of virulence was investigated by comparing photocatalytic destruction of a biofilm producing and non-producing strain of Staphylococcus epidermidis. The results indicated that there were no differences in susceptibility to disinfection suggesting that the capacity alone to express a virulence factor may not generate greater resistance to photocatalytic destruction.