Directly Quantifiable Biotinylation Using a Water-Soluble Isatoic Anhydride Platform
Directly Quantifiable Biotinylation Using a Water-Soluble Isatoic Anhydride Platform
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DOI：
10.1021/acs.bioconjchem.1c00150
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发表时间：
2021-04-21
影响因子：
4.7
通讯作者：
Ogle, Craig A.
中科院分区：
文献类型：
--
作者：
Fessler, Adam B.;Fowler, Anthony J.;Ogle, Craig A.
靛红酸酐（IA）已被证明是用于可量化的生物缀合的有用平台。具有生物素的基于水溶性靛红酸酐的平台的制备通过非破坏性定量方法提供了易于定量的生物素化试剂。功能性的掺入使用位于生物窗口之外的试剂的独特吸光度或荧光特征直接定量。几种生物素化试剂制备不同的接头长度，和生物素化的蛋白质的定量进行了证明，并从传统的HABA测定的结果进行比较。
Isatoic anhydride (IA) has been shown to be a useful platform for quantifiable bioconjugation. The elaboration of a water-soluble isatoic anhydride-based platform with biotin offers readily quantifiable biotinylation reagents through nondestructive methods of quantification. The incorporation of functionality is directly quantified using the reagent's unique absorbance or fluorescence signature, located outside the biological window. Several biotinylation reagents are prepared with various linker lengths, and the quantification of biotinylated proteins is demonstrated and compared to results from the traditional HABA assay.