PLGA/PLA micro- and nanoparticle formulations serve as antigen depots and induce elevated humoral responses after immunization of Atlantic salmon (Salmo salar L.)
PLGA/PLA micro- and nanoparticle formulations serve as antigen depots and induce elevated humoral responses after immunization of Atlantic salmon (Salmo salar L.)
复制标题
DOI：
10.1016/j.vaccine.2011.10.105
复制
发表时间：
2012-01-11
期刊：
影响因子：
5.5
通讯作者：
Grip, Jostein
中科院分区：
文献类型：
--
作者：
Fredriksen, Borge N.;Grip, Jostein
为了改善鲑鱼养殖业，迫切需要新的疫苗输送系统。尽管聚乳酸-乙醇酸(PLGA)等生物相容聚合物颗粒长期以来一直被认为是传递免疫原化合物的有前途的候选材料，但很少有人研究它们作为疫苗载体在大西洋鲑鱼(Salmo Solar L)中的应用。为了研究它们保留/储存抗原的能力，以及诱导时间和剂量依赖的适应性体液免疫反应的能力，我们在这里对PLGA和PLA颗粒在鲑鱼疫苗中可能具有的佐剂特性进行了基础研究。将模型抗原(人丙种球蛋白，HGG)与β-葡聚糖共包裹在纳米颗粒(
Novel vaccine delivery systems are highly needed to improve the salmon aquaculture industry. Although particles of biocompatible polymers such as poly(lactic-co-glycolic) acid (PLGA) have long been considered promising candidates for delivery of immunogenic compounds, few studies have addressed their use as vaccine carriers in Atlantic salmon (Salmo solar L). Investigating their ability to retain/depot antigen and induce time and dosage dependent adaptive humoral responses to immunization, we here present a basic study of the adjuvantic properties PLGA and PLA particles may have in salmon vaccines. A model antigen (human gamma globulin, HGG) was co-encapsulated with p-glucan in nanoparticles (