Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging -: art. no. 041207
Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging -: art. no. 041207
复制标题
DOI：
10.1117/1.2032458
复制
发表时间：
2005-07-01
影响因子：
3.5
通讯作者：
Levenson, RM
中科院分区：
文献类型：
--
作者：
Mansfield, JR;Gossage, KW;Levenson, RM
体内荧光标记标记的成像和定量能力通常受到组织自发荧光的限制。尤其是皮肤，具有很强的自发荧光信号，特别是在蓝色或绿色波长下激发时。发射波长在近红外范围内的荧光标记更适合深层组织成像，因为随着波长的增加，散射和自发荧光都会减少，但即使在这些光谱区域，自发荧光仍然会限制灵敏度。然而，多光谱成像 (MSI) 可以消除自发荧光引起的信号衰减，同时增强多路复用功能。虽然光谱“库”的可用性使得对特征良好的样品进行多光谱分析成为常规，但新的软件工具已经开发出来，大大简化了 MSI 在新样品中的应用。 (C) 2005 年光电仪器工程师协会。
The ability to image and quantitate fluorescently labeled markers in vivo has generally been limited by autofluorescence of the tissue. Skin, in particular, has a strong autofluorescence signal, particularly when excited in the blue or green wavelengths. Fluorescence labels with emission wavelengths in the near-infrared are more amenable to deep-tissue imaging, because both scattering and autofluorescence are reduced as wavelengths are increased, but even in these spectral regions, autofluorescence can still limit sensitivity. Multispectral imaging (MSI), however, can remove the signal degradation caused by autofluorescence while adding enhanced multiplexing capabilities. While the availability of spectral "libraries" makes multispectral analysis routine for well-characterized samples, new software tools have been developed that greatly simplify the application of MSI to novel specimens. (C) 2005 Society of Photo-Optical Instrumentation Engineers.