Control of Nutrient Solution pH with an Ion Exchange System: Effect on Soybean Nodulation
Control of Nutrient Solution pH with an Ion Exchange System: Effect on Soybean Nodulation
复制标题
用离子交换系统控制营养液 pH 值：对大豆结瘤的影响
DOI：
10.1111/j.1399-3054.1976.tb04852.x
复制
发表时间：
1976
影响因子：
6.4
通讯作者：
J. C. Nicholas
中科院分区：
文献类型：
--
作者：
J. E. Harper;J. C. Nicholas
大豆（Glycine max（L.）梅尔）在溶液培养中，由于溶液pH值的不均匀变化而使不同单一氮源之间的相互作用受到干扰。设计了一种循环离子交换系统，其中使用阳离子交换树脂（Amberlite IRC 50）来控制大豆生长溶液的pH值。营养液的pH值水平分别在9.0至3.7的极端范围内，100%Ca2+或H+形式的树脂。通过改变树脂的Ca2+与H+形式的比率来建立中间pH水平。该系统是能够保持在0.5至0.9单位的初始pH值超过两个星期的生长期的大豆与硝酸盐或尿素氮源的pH值。在没有树脂柱的情况下，尿素营养液的pH迅速下降至小于pH 4，这导致植物根瘤发育受到抑制。根瘤质量和N2固定（乙炔还原测定）的最佳pH值范围为5.2和7.0之间的尿素营养。硝酸盐和铵态氮源抑制乙炔还原尿素相比，允许广泛的结核的发展和活动。
Growth and nodulation response of soybean (Glycine max (L.) Merr.) to various single nitrogen sources in solution culture is confounded by unequal shifts in solution pH. A recirculating ion exchange system was designed in which a cation exchange resin (Amberlite IRC 50) was used to control the pH of solutions in which soybeans were grown. Nutrient solution pH levels were established at range extremes of 9.0 to 3.7 with 100% Ca2+ or H+ forms of resin, respectively. Intermediate pH levels were established by varying the ratio of Ca2+ to H+ forms of resin. The system is capable of maintaining pH within 0.5 to 0.9 units of the initial pH over a two-week growth period of soybeans with either nitrate- or urea-N sources. In the absence of the resin column, pH of the urea nutrient solution rapidly declined to less than pH 4 which resulted in depressed plant nodule development. The optimum pH range for nodule mass and N2 fixation (measured by acetylene reduction) was between 5.2 and 7.0 with urea nutrition. Both nitrate- and ammonium-N sources were inhibitory to acetylene reduction in comparison with urea which allowed extensive nodule development and activity.