Uncoupling protein 1 dependent reactive oxygen species production by thymus mitochondria
Uncoupling protein 1 dependent reactive oxygen species production by thymus mitochondria
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DOI：
10.1016/j.biocel.2012.09.023
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发表时间：
2013-01-01
影响因子：
4
通讯作者：
Porter, Richard K.
中科院分区：
文献类型：
--
作者：
Clarke, Kieran J.;Porter, Richard K.
我们之前已经证明，解偶联蛋白1存在于胸腺中，并在T细胞发育中发挥作用。由于活性氧类与T细胞发育有关，我们着手确定解偶联蛋白1是否有可能调节胸腺线粒体中活性氧类的产生。这是通过使用嘌呤核苷酸GDP抑制解偶联蛋白1的质子泄漏，以及通过消融解偶联蛋白1，测量线粒体产生Amplex Red敏感的活性氧物种来实现的。在这项工作中，我们首次证明了解偶联蛋白1具有调节胸腺线粒体中活性氧产生的潜力。我们还发现，胸腺线粒体在琥珀酸和甘油-3-磷酸上呼吸的反向电子传递是可能的。解偶联蛋白1的这种调节作用的含义在胸腺功能的背景下进行了讨论。这篇文章是题为：生物能量功能障碍、适应和治疗的定向问题的一部分。(C)2012爱思唯尔有限公司。保留所有权利。
We have previously shown that uncoupling protein 1 is present in thymus and has a role in T-cell development. As reactive oxygen species have been implicated in T-cell development, we set out to determine whether uncoupling protein 1 had the potential to regulate reactive oxygen species production in mitochondria isolated from thymus. This was achieved by inhibiting proton leak through uncoupling protein 1 using the purine nucleotide GDP and through ablation of uncoupling protein 1, measuring the amplex red sensitive reactive oxygen species production by mitochondria. In this work we demonstrate, for the first time, that uncoupling protein 1 has the potential to regulate reactive oxygen species production in thymus mitochondria. We also show that reverse electron transport is possible in thymus mitochondria respiring on succinate and glycerol-3-phosphate. The implications of this regulatory role for uncoupling protein 1 are discussed in the context of thymus function.This article is part of a Directed Issue entitled: Bioenergetic dysfunction, adaptation and therapy. (C) 2012 Elsevier Ltd. All rights reserved.