Overexpression of FMNL2 is closely related to metastasis of colorectal cancer
Overexpression of FMNL2 is closely related to metastasis of colorectal cancer
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FMNL2过表达与结直肠癌转移密切相关
DOI：
10.1007/s00384-008-0520-2
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发表时间：
2008-11-01
影响因子：
2.8
通讯作者：
Ding, Yan-Qing
中科院分区：
文献类型：
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作者：
Zhu, Xi-Ling;Liang, Li;Ding, Yan-Qing
背景与目的形成素样蛋白2（Formin-like 2，FMNL 2）是透明相关形成素家族的一员，参与肌动蛋白依赖的细胞运动和侵袭等过程。在这项研究中，我们研究了FMNL 2在结直肠癌（CRC）中的表达及其与CRC转移的相关性。患者-方法石蜡包埋的CRC标本（包括75个原发性CRC肿瘤和45个相应的转移淋巴结）和正常结直肠粘膜（30个样本）用FMNL 2抗体进行免疫染色。对32对速冻肿瘤组织和邻近正常结直肠粘膜进行实时逆转录聚合酶链反应（RT-PCR）。通过Western印迹和实时RT-PCR测定六种CRC细胞系（SW 480、SW 620、SW 480/M5、LoVo、LS 174 T和HT 29）的FMNL 2表达。结果FMNL 2在结直肠癌组织及相应转移淋巴结中的表达明显高于正常大肠粘膜（P<0.0 5）。FMNL 2表达与大肠癌淋巴结转移密切相关（P< 0.05）。实时RT-PCR分析证实了免疫组化获得的结果。在mRNA和蛋白质水平上，与源自原发性CRC的细胞系（HT 29、LS 174 T和SW 480;P< 0.05）相比，源自CRC转移的细胞系（SW 620、SW 480/M5和LoVo）中FMNL 2表达显著上调。结论FMNL 2在大肠癌转移过程中可能起重要作用，可作为大肠癌转移的一个有用的标志物。
Background and aimsFormin-like 2 (FMNL2) is a member of diaphanous-related formins which can control the actin-dependent processes such as cell motility and invasion. In this study, we investigated the expression of FMNL2 in colorectal cancer (CRC) and its correlation with CRC metastasis.Patients–methodsParaffin-embedded specimens of CRC (including 75 primary CRC tumors and 45 corresponding metastatic lymph nodes) and normal colorectal mucosa (30 samples) were immunostained with a FMNL2 antibody. Thirty-two paired snap-frozen tumor tissues and adjacent normal colorectal mucosa were subjected to real-time reverse-transcription polymerase chain reaction (RT-PCR). Six CRC cell lines (SW480, SW620, SW480/M5, LoVo, LS174T, and HT29) were assayed for FMNL2 expression by Western blotting and real-time RT-PCR. Their invasive abilities in vitro were determined by Boyden chamber assay.ResultsThe immunohistochemical analysis showed FMNL2 expression was considerably higher in CRC tumors and corresponding metastatic lymph nodes than in normal colorectal mucosa (P< 0.05, respectively). Elevated FMNL2 expression was significantly correlated with lymphatic metastasis of CRC (P< 0.05). Real-time RT-PCR analysis confirmed the results obtained by immunohistochemistry. At mRNA and protein levels, FMNL2 expression was substantially upregulated in cell lines derived from CRC metastases (SW620, SW480/M5, and LoVo) compared to ones derived from primary CRC (HT29, LS174T, and SW480;P< 0.05). In vitro cell invasive assay demonstrated that the former three cell lines had higher invasive ability than the latter cell lines.ConclusionsFMNL2 may play an important role in the metastasis of CRC and may be a useful marker for metastasis of CRC.