A preliminary investigation of the effects of arsenate on irradiation-induced DNA damage in cultured human lung fibroblasts
A preliminary investigation of the effects of arsenate on irradiation-induced DNA damage in cultured human lung fibroblasts
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DOI：
10.1080/152873901316857789
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发表时间：
2001-08-01
期刊：
影响因子：
--
通讯作者：
Gould, D
中科院分区：
文献类型：
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作者：
Curnow, A;Salter, L;Gould, D
单细胞凝胶电泳（彗星试验）用于评估单独氙灯照射、不同浓度的砷酸盐[As（V）]单独或两者的各种组合在培养的人肺成纤维细胞中产生的单链断裂（SSB）。结果发现，显着更高水平的SSB中观察到的照射细胞比non-radiated细胞和砷的水平升高，提高了检测到的损伤水平在照射和non-radiated细胞中的浓度依赖性的方式，也就是说，单独培养细胞与砷酸盐产生显着的DNA损伤没有辐射损伤是必要的。这项研究的结果表明，砷酸盐是作为一个cogenotoxin与辐射在这个细胞系。这种叠加效应也可能是共致癌的，因此，在砷暴露人群中，可能需要较少的太阳辐射才能诱发皮肤癌。
Single-cell gel electrophoresis ( the comet assay) was used to assess single-strand breaks (SSBs) produced in cultured lung human fibroblasts by xenon lamp irradiation alone, various concentrations of arsenate [As(V)], alone or various combinations of the two. It was found that significantly higher levels of SSBs were observed in the irradiated cells than the nonirradiated cells and that elevating levels of arsenate enhanced the level of damage detected in both irradiated and nonirradiated cells in a concentration-dependent manner; that is, incubating cells with arsenate alone produced marked DNA damage without an irradiation insult being necessary. The results of this study indicate that arsenate is acting as a cogenotoxin with irradiation in this cell line. This additive effect may also be cocarcinogenic, and as a result it is possible that less solar irradiation may be required to induce skin cancer in arsenic-exposed populations.