PRODUCTION OF BASEMENT-MEMBRANE COMPONENTS BY A RECONSTRUCTED EPIDERMIS CULTURED IN THE ABSENCE OF SERUM AND DERMAL FACTORS
PRODUCTION OF BASEMENT-MEMBRANE COMPONENTS BY A RECONSTRUCTED EPIDERMIS CULTURED IN THE ABSENCE OF SERUM AND DERMAL FACTORS
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DOI：
10.1111/j.1365-2133.1993.tb11839.x
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发表时间：
1993-09-01
影响因子：
10.3
通讯作者：
ORTONNE, JP
中科院分区：
文献类型：
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作者：
ROSDY, M;PISANI, A;ORTONNE, JP
通过在规定的培养基和惰性聚碳酸酯过滤基质中培养第二代正常人角质形成细胞14天，在体外获得了具有人表皮特征的完全分化上皮。组织学和间接免疫荧光染色的垂直切片显示，“基底”细胞合成并分泌半脂质体和透明层的所有主要标记物。层密度的组成也被表示出来。VII型胶原是合成的，但不是分泌的。超微结构研究显示，半粒内可见密集斑块和锚定细丝，基底膜样结构清晰可见。这些结果表明，在没有血清和真皮因子的情况下，表皮细胞能够产生半脂粒并分泌真皮-表皮交界处的主要成分，这表明基底膜的合成和半脂粒的组装并不依赖于真皮的存在。
A fully differentiated epithelium displaying features of human epidermis was obtained in vitro by culturing second-passage normal human keratinocytes for 14 days in defined medium and on an inert polycarbonate filter substratum at the air-liquid interface.Vertical sections stained for histology and indirect immunofluorescence studies showed that the 'basal' cells synthesize and secrete all major markers of hemidesmosomes and the lamina lucida. Components of the lamina densa are also expressed. Collagen VII is synthesized, but not secreted. Ultrastructural studies showed the presence of hemidesmosomes with major dense plaques and anchoring filaments, and a basement membrane-like structure was clearly identified.These results show that epidermal cells are able to produce hemidesmosomes and to secrete the major components of the dermo-epidermal junction in the absence of serum and dermal factors, suggesting that basement membrane synthesis and hemidesmosome assembly are not dependent on the presence of dermis.