Changes in acid-phosphate content in enamel mineral during porcine amelogenesis.
Changes in acid-phosphate content in enamel mineral during porcine amelogenesis.
复制标题
猪釉质形成过程中牙釉质矿物质中酸式磷酸盐含量的变化。
DOI：
10.1177/00220345910700120801
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发表时间：
1991
影响因子：
7.6
通讯作者：
Moreno,EC
中科院分区：
文献类型：
--
作者：
Shimoda,S;Aoba,T;Moreno,EC
本研究旨在探讨猪牙釉质矿物质在其发育过程中酸性磷酸盐含量的变化，并分别评估不稳定和稳定形式的HPO 42-池。从5 - 6月龄屠宰仔猪的恒切牙中获得了釉质发生的分泌期和成熟期的釉质样品。包括来自萌出的、拔除的牙齿、合成羟基磷灰石和含有酸性磷酸盐的碳酸磷灰石的人牙釉质作为参考。每个样品的酸式磷酸盐含量通过其热解转化为焦磷酸盐来化学测定。通过分析用含有3 mmol/L磷酸盐的pH 111的溶液预平衡的样品（以使晶体表面上的HPO 42-物质去质子化）来进行不稳定形式的HPO 42-的评估。猪牙釉质样品的分析结果表明：（a）最外层的分泌（B）酸性磷酸盐含量逐渐下降，内分泌期为10%，成熟期为6%;（c）大部分HPO 42-在发育中的釉质组织中（50-60%的分泌釉HPO_（42-））为不稳定型;（d）随着釉质矿物质的生长，不稳定的HPO_（42-）库减少。在对成熟人釉质的平行研究中，确定了总的酸性磷酸盐仅为总磷酸盐的约3%，远低于发育中的猪牙釉质，并且不稳定的HPO 42-库也很小，对应于测定的总酸性磷酸盐的约15%。结果表明，酸性磷酸盐是釉质形成过程中形成的早期釉质晶体的主要成分之一，生长中的釉质晶体表面富含HPO_（42-）。
The present study was undertaken to investigate changes in the acid-phosphate content of porcine enamel mineral during its development and to assess separately the HPO42-pools in labile and stable forms. Enamel samples at the secretory and maturing stages of amelogenesis were obtained from the permanent incisors of five- to six-month-old slaughtered piglets. Human enamel from erupted, extracted teeth, synthetic hydroxyapatite, and carbonatoapatite containing acid phosphate were included as references. The acid-phosphate content of each sample was determined chemically through its pyrolytic conversion to pyrophosphate. The assessment of HPO42-in labile forms was made by analysis of samples pre-equilibrated with solutions containing 3 mmol/L phosphate at pH 11 1 (to de-protonate the HPO42-species on crystal surfaces). The analytical results of porcine enamel samples showed that: (a) the outermost secretory (youngest) enamel contained the highest HPO42-, corresponding to about 16% of the total phosphate; (b) the acid-phosphate content decreased gradually to 10% in the inner (older) secretory and to 6% in the maturing tissue; (c) a substantial part of the HPO42-in developing enamel tissue (50-60% of the HPO42-for the secretory enamel) was in labile forms; and (d) the pool of the labile HPO42-decreased with the growth of enamel mineral. In parallel studies with mature human enamel, it was ascertained that the total acid phosphate was only about 3% of the total phosphate, much lower than in developing porcine enamel, and that the labile pool of HPO42-was also small, corresponding to about 15% of the total acid phosphate determined. The overall results indicate that acid phosphate is one of the major constituents of the early enamel crystals formed during amelogenesis and that the surfaces of the growing enamel crystallites are rich in HPO42-.