THE TRANSLATION MACHINERY AND 70 KD HEAT-SHOCK PROTEIN COOPERATE IN PROTEIN-SYNTHESIS
THE TRANSLATION MACHINERY AND 70 KD HEAT-SHOCK PROTEIN COOPERATE IN PROTEIN-SYNTHESIS
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DOI：
10.1016/0092-8674(92)90269-i
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发表时间：
1992-10-02
期刊：
影响因子：
64.5
通讯作者：
CRAIG, EA
中科院分区：
文献类型：
--
作者：
NELSON, RJ;ZIEGELHOFFER, T;CRAIG, EA
采用多种方法研究了酵母SSB 70 kd热休克蛋白（hsp 70 s）的功能。SSB hsp 70（Ssb 1/2 p）与翻译核糖体相关。嘌呤霉素破坏了这种关联，表明Ssb 1/2 p可能直接与新生多肽结合。突变型ssb 1 ssb 2菌株生长缓慢，含有少量的翻译核糖体，对几种蛋白质合成抑制剂过敏。ssb 1和ssb 2突变体的缓慢生长表型受到编码新型翻译延伸因子1 α（EF-1 α）样蛋白的基因拷贝数增加的抑制。我们认为，胞质热休克蛋白70艾滋病在新生的多肽链通过核糖体的方式类似的细胞器本地化的热休克蛋白70在跨膜的蛋白质运输中所发挥的作用。
The function of the yeast SSB 70 kd heatshock proteins (hsp70s) was investigated by a variety of approaches. The SSB hsp70s (Ssb1/2p) are associated with translating ribosomes. This association is disrupted by puromycin, suggesting that Ssb1/2p may bind directly to the nascent polypeptide. Mutant ssb1 ssb2 strains grow slowly, contain a low number of translating ribosomes, and are hypersensitive to several inhibitors of protein synthesis. The slow growth phenotype of ssb1 ssb2 mutants is suppressed by increased copy number of a gene encoding a novel translation elongation factor 1alpha (EF-1alpha)-like protein. We suggest that cytosolic hsp70 aids in the passage of the nascent polypeptide chain through the ribosome in a manner analogous to the role played by organelle-localized hsp70 in the transport of proteins across membranes.