Isolation of a novel rmn1 gene genetically linked to spnab2 with respect to mRNA export in fission yeast
Isolation of a novel rmn1 gene genetically linked to spnab2 with respect to mRNA export in fission yeast
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DOI：
10.1007/s10059-012-0157-4
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发表时间：
2012-09-01
影响因子：
3.8
通讯作者：
Yoon, Jin Ho
中科院分区：
文献类型：
--
作者：
Cho, Ye-Seul;Jang, Sooyeon;Yoon, Jin Ho
在裂殖酵母裂殖酵母中，spnab 2基因编码芽殖酵母核丰富的多聚（A）（+）RNA结合蛋白2（Nab 2）的直系同源物，Nab 2是mRNA生物合成和mRNA向细胞质的核输出所需的必需蛋白。我们以前已经分离出三个突变体（SLnab 1 -3），显示合成的spnab 2表达的抑制条件下的致死性。在这项研究中，我们分离出一种新的rmn 1基因作为一个多拷贝抑制，补充SLnab 1突变细胞的生长和mRNA输出的缺陷。rmn 1基因含有3个内含子，编码一个589个氨基酸的蛋白质，其中心区域具有RNA识别基序（RRM）。Δ rmn 1无效突变体是可行的，但表现出s轻mRNA输出缺陷。然而，它的过度表达导致了对生长的有害影响，伴随着poly（A）（+）RNA在细胞核中的强烈积累。Delta rmn 1与Delta spnab 2或Delta spmex 67的组合也抑制生长。此外，Rmn 1 p在体内与Rae 1 p相关。这些结果表明，rmn 1是一个新的基因，在功能上与spnab 2。
In fission yeast, Schizosaccharomyces pombe, the spnab2 gene encodes an ortholog of the budding yeast nuclear abundant poly(A)(+) RNA-binding protein 2 (Nab2) that is an essential protein required for both mRNA biogenesis and nuclear export of mRNA to the cytoplasm. We have previously isolated three mutants (SLnab1-3) that showed synthetic lethality under the repressed condition of spnab2 expression. In this study, we isolated a novel rmn1 gene as a multicopy suppressor that complemented the defects in growth and mRNA export of SLnab1 mutant cells. The rmn1 gene contained three introns and encoded a 589 amino-acid protein with the RNA recognition motif (RRM) in the central region. The Delta rmn1 null mutant was viable but showed a s light mRNA export defect. However, its over-expression caused a deleterious effect on growth accompanied by intense accumulation of poly(A)(+) RNA in the nucleus. The combination of Delta rmn1 with Delta spnab2 or Delta spmex67 also inhibited growth. In addition, Rmn1p was associated with Rae1p in vivo. These results suggest that rmn1 is a novel gene that is functionally linked to spnab2.