Polyclonal anti-propylbenzilylcholine mustard antibodies selectively recognize labeled muscarinic receptors from rabbit brain.
Polyclonal anti-propylbenzilylcholine mustard antibodies selectively recognize labeled muscarinic receptors from rabbit brain.
复制标题
多克隆抗丙基苯甲酰胆碱芥子抗体选择性识别兔脑中标记的毒蕈碱受体。
DOI：
10.1016/0304-3940(89)90355-8
复制
发表时间：
1989
影响因子：
2.5
通讯作者：
Flynn,DD
中科院分区：
文献类型：
--
作者：
Strang,PF;Flynn,DD
制备了兔抗丙基苄胆碱芥-牛血清白蛋白（PBCM-BSA）结合物的多克隆抗体。抗体选择性地识别并结合到对PBCM具有高亲和力的明显单一类别的位点。抗体还显示出对具有与PBCM类似的二苯甲基或二环己环结构的毒蕈碱配体的高至中等亲和力。抗体对缺乏双环己烷结构的配体具有很少或没有亲和力。在免疫印迹上，抗体识别用[3 H]PBCM特异性标记的毒蕈碱受体，但不识别未标记的受体。这些结果表明，在这项研究中产生的多克隆抗体反应特异性与PBCM上的二环己烷结构，该抗原决定簇仍然可用于与抗体的相互作用，即使当配体结合到受体。
Polyclonal antibodies were produced in rabbits to propylbenzilylcholine mustard-bovine serum albumin (PBCM-BSA) conjugates. The antibodies selectively recognized and bound to an apparent single class of sites with high affinity for PBCM. The antibodies also demonstrated high to intermediate affinity for muscarinic ligands which have benzhydryl or dicyclohexane ring structures similar to PBCM. The antibodies had little or no affinity for ligands which lacked the double cyclohexane structure. Antibodies recognized muscarinic receptors specifically labeled with [3H]PBCM, but not unlabeled receptors, on immunoblots. These results suggest that the polyclonal antibodies produced in this study react specifically with the dicyclohexane structure on PBCM and that this antigenic determinant remains available for interaction with the antibodies, even when the ligand is bound to the receptor.