New Techniques for Qligosaccharide Sequencing
New Techniques for Qligosaccharide Sequencing
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寡糖测序新技术
DOI：
10.1080/07328308308058806
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发表时间：
1983
影响因子：
1
通讯作者：
S. Carr
中科院分区：
文献类型：
--
作者：
V. Reinhold;E. Coles;S. Carr
摘要建立了一种结合高效液相色谱（HPLC）纯化、荧光检测敏感技术、离子氢化部分聚合物降解以及直接化学电离质谱（DCI-MS）和气相色谱-质谱（GC-MS）测定成分的低聚糖测序新方法。该方法证明了一系列的模型化合物，并被证明提供链接信息以及糖序列。
Abstract A new procedure is described for oligosaccharide sequencing that combines HPLC purification, the sensitive technique of fluorescence detection, and partial polymer degradation by ionic hydrogenation with component determination by direct chemical ionization mass spectrometry (DCI-MS) and GC-MS. The methodology is demonstrated for a series of model compounds and is shown to provide linkage information as well as sugar sequence.