Metabolic remodeling of the human red blood cell membrane
Metabolic remodeling of the human red blood cell membrane
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DOI：
10.1073/pnas.0910785107
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发表时间：
2010-01-26
影响因子：
11.1
通讯作者：
Feld, Michael S.
中科院分区：
文献类型：
--
作者：
Park, YongKeun;Best, Catherine A.;Feld, Michael S.
人红细胞（RBC）的显著变形性是由磷脂双层和血影蛋白分子网络的耦合动力学响应引起的。在这里，我们提出了定量的血影蛋白形态和膜波动的人红细胞之间的联系，通过使用动态全场激光干涉技术。我们提出了确凿的证据表明，腺苷5 '-三磷酸（ATP）的存在下，促进红细胞膜的非平衡动态波动，这是高度相关的红细胞的双凹形状。波动的空间分析表明，这些非平衡膜振动增强的spectrin网格大小的规模。我们的研究结果表明，由ATP驱动的耦合膜的动态重塑导致非平衡膜波动，体现从代谢和热能，也保持了红细胞的双凹形状。
The remarkable deformability of the human red blood cell (RBC) results from the coupled dynamic response of the phospholipid bilayer and the spectrin molecular network. Here we present quantitative connections between spectrin morphology and membrane fluctuations of human RBCs by using dynamic full-field laser interferometry techniques. We present conclusive evidence that the presence of adenosine 5'-triphosphate (ATP) facilitates non-equilibrium dynamic fluctuations in the RBC membrane that are highly correlated with the biconcave shape of RBCs. Spatial analysis of the fluctuations reveals that these non-equilibrium membrane vibrations are enhanced at the scale of spectrin mesh size. Our results indicate that the dynamic remodeling of the coupled membranes powered by ATP results in non-equilibrium membrane fluctuations manifesting from both metabolic and thermal energies and also maintains the biconcave shape of RBCs.