The metabolic activation of chrysene by hamster embryo cells.
The metabolic activation of chrysene by hamster embryo cells.
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仓鼠胚胎细胞对屈的代谢激活。
DOI：
10.1093/carcin/3.9.1051
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发表时间：
1982
期刊：
影响因子：
4.7
通讯作者：
Sims,P
中科院分区：
文献类型：
--
作者：
Hodgson,RM;Pal,K;Grover,PL;Sims,P
用SephadexLH 20柱层析和高效液相色谱法对金黄地鼠胚胎细胞DNA水解产物中的主要脱氧核糖核苷-烃加合物进行了分析。Zorbax ODS上结果表明，这两种主要加合物的色谱性质与r-1，t-2-dihydroxy-t-3，4-oxy-1，2，3，4-tetrahydrochyrrhenes与DNA反应时所形成的加合物的色谱性质相同，并提供了证据，证明在这种“海湾区域”二醇环氧化物的形成过程中，发生了chyrrhenes的代谢活化。
The major deoxyribonudeoside—hydrocarbon adducts present in hydrolysates of DNA isolated from hamster embryo cells treated with chrysene were examined by chromatography on Sephadex LH20 and by h.p.l.c. on Zorbax ODS. The results show that both major adducts have chromatographic properties identical to those of adducts formed whenr-1,t-2-dihydroxy-t-3,4-oxy-1,2,3,4-tetrahydrochrysene reacts with DNA and provide evidence that metabolic activation of chrysene occursviathe formation of this ‘bay-region’ diolepoxide.